ISOLATION AND COMPOSITION OF THE EXTRACELLULAR SLIME MADE BY COAGULASE-NEGATIVE STAPHYLOCOCCI IN A CHEMICALLY DEFINED MEDIUM

被引:57
作者
HUSSAIN, M [1 ]
HASTINGS, JGM [1 ]
WHITE, PJ [1 ]
机构
[1] UNIV SHEFFIELD,DEPT MOLEC BIOL & BIOTECHNOL,WESTERN BANK,SHEFFIELD S10 2TN,S YORKSHIRE,ENGLAND
关键词
D O I
10.1093/infdis/163.3.534
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Slime isolated after growth of four strains of coagulase-negative staphylococci on chemically defined medium plus agar was rich in galactose. However, when sterile agar plates were extracted with saline, high-molecular-weight material with similar properties was obtained that also was galactose-rich. Most of the dry weight attributed to slime, and probably all the galactose, originated from agar. Slime isolated by gel and ion-exchange chromatography from liquid culture in the same medium contained glycerol phosphate, glucose (no galactose), glucosamine, alanine, uronate, an unidentified component, and protein. Separation of protein from carbohydrate was achieved by affinity chromatography. [C-14]glucose in the medium labeled the carbohydrate polymer; [C-14]amino acids chiefly labeled extracellular proteins. Slime from bacteria grown on medium solidified with silica gel or on dialysis membrane above an agar surface showed essentially the same composition and behavior after purification as the material isolated from liquid culture.
引用
收藏
页码:534 / 541
页数:8
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