AMINES PROTECT INVITRO THE CELIAC SMALL-INTESTINE FROM THE DAMAGING ACTIVITY OF GLIADIN PEPTIDES

被引:20
作者
AURICCHIO, S
DERITIS, G
DEVINCENZI, M
GENTILE, V
MAIURI, L
MANCINI, E
PORTA, R
RAIA, V
机构
[1] NAPLES UNIV,FAC MED & CHIRURG 2,DEPT BIOCHEM & BIOPHYS,I-80131 NAPLES,ITALY
[2] CNR,PROJECT PERINATAL PATHOL & CONSEQUENCES,IST SCI ALIMENTAT,ROME,ITALY
[3] CNR,PROJECT PERINATAL PATHOL & CONSEQUENCES,PROGRAMME PREVENT MED,ROME,ITALY
[4] IST SUPER SANITA,DEPT METAB & PATHOL BIOCHEM,I-00161 ROME,ITALY
关键词
D O I
10.1016/0016-5085(90)90473-E
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Proteins and peptides responsible for the celiac small intestinal lesion inhibit both the enterocyte recovery of in vitro cultured flat celiac mucosa and the in vitro development of fetal rat intestine. They also agglutinate K 562 (S) cells. Using these three in vitro systems (cultured human celiac and rat fetal intestine and cell agglutination), it is shown that several small-molecular-weight amines, mostly the polyamines spermidine and spermine, prevent and reverse K 562 (S) cell agglutination induced by gliadin peptides, whereas they do not prevent cell agglutination induced by concanavalin A and wheat germ agglutinin. Some of these amines also protected in vitro developing fetal rat intestine and flat celiac mucosa from the damaging effect of gliadin peptides. This protective effect may be related to the trophic activity exerted by amines on the intestine and/or the effect of amines on the functions of intestinal brush border or intracellular membranes involved in the intestinal handling of gliadins. © 1990.
引用
收藏
页码:1668 / 1674
页数:7
相关论文
共 56 条
  • [11] THE INCORPORATION OF AMINES INTO PROTEIN
    CLARKE, DD
    MYCEK, MJ
    NEIDLE, A
    WAELSCH, H
    [J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1959, 79 : 338 - 354
  • [12] DEMBINSKI AB, 1984, AM J PHYSIOL, V274, pG352
  • [13] DERITIS G, 1984, PEDIATR RES, V18, P1052
  • [14] INVITRO (ORGAN-CULTURE) STUDIES OF THE TOXICITY OF SPECIFIC A-GLIADIN PEPTIDES IN CELIAC-DISEASE
    DERITIS, G
    AURICCHIO, S
    JONES, HW
    LEW, EJL
    BERNARDIN, JE
    KASARDA, DD
    [J]. GASTROENTEROLOGY, 1988, 94 (01) : 41 - 49
  • [15] DERITIS G, 1979, PEDIATR RES, V13, P1255
  • [16] DERITIS G, 1982, DEV BIOL, V45, P304
  • [17] DERITIS G, 1988, PEDIATR RES, V24, P412
  • [18] SPERMINE AND SPERMIDINE INDUCE INTESTINAL MATURATION IN THE RAT
    DUFOUR, C
    DANDRIFOSSE, G
    FORGET, P
    VERMESSE, F
    ROMAIN, N
    LEPOINT, P
    [J]. GASTROENTEROLOGY, 1988, 95 (01) : 112 - 116
  • [19] POLYAMINES STIMULATE D-GLUCOSE TRANSPORT IN ISOLATED RENAL BRUSH-BORDER MEMBRANE-VESICLES
    ELGAVISH, A
    WALLACE, RW
    PILLION, DJ
    MEEZAN, E
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 777 (01) : 1 - 8
  • [20] IN-VITRO MODEL OF GLUTEN-SENSITIVE ENTEROPATHY - EFFECT OF GLIADIN ON INTESTINAL EPITHELIAL-CELLS OF PATIENTS WITH GLUTEN-SENSITIVE ENTEROPATHY IN ORGAN-CULTURE
    FALCHUK, ZM
    GEBHARD, RL
    SESSOMS, C
    STROBER, W
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1974, 53 (02) : 487 - 500