CROSS-LINKING PROTEINS TO NUCLEIC-ACIDS BY ULTRAVIOLET-LASER IRRADIATION

被引：95

作者：

PASHEV, IG ^{[1
]}

DIMITROV, SI ^{[1
]}

ANGELOV, D ^{[1
]}

机构：

[1] BULGARIAN ACAD SCI,INST SOLID STATE PHYS,BU-1113 SOFIA,BULGARIA

来源：

TRENDS IN BIOCHEMICAL SCIENCES | 1991年 / 16卷 / 09期

关键词：

D O I：

10.1016/0968-0004(91)90133-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ultraviolet (UV) irradiation can initiate complex formation between proteins and DNA or RNA and so can be used to study such interactions. However, crosslink formation by standard UV light sources can take up to several hours. More recently, a beam of monochromatic UV light from a laser has been used to initiate crosslinking in nano- and picosecond time intervals. As noted in an earlier TIBS article 'the advantages of short pulse times and high-energy fluxes should make this a valuable technique in the future' 1. In this review we characterize laser-induced crosslinking and explore the applications of this method.

引用

页码：323 / 326

页数：4

共 19 条

[1] CROSS-LINKING OF DEOXYRIBONUCLEIC ACID TO PROTEIN FOLLOWING ULTRA-VIOLET IRRADIATION OF DIFFERENT CELLS [J].

ALEXANDER, P ;

MOROSON, H .

NATURE, 1962, 194 (4831) :882-&

[2] PROTEIN DNA CROSSLINKING IN RECONSTITUTED NUCLEOHISTONE, NUCLEI AND WHOLE CELLS BY PICOSECOND UV LASER IRRADIATION [J].

ANGELOV, D ;

STEFANOVSKY, VY ;

DIMITROV, SI ;

RUSSANOVA, VR ;

KESKINOVA, E ;

PASHEV, IG .

NUCLEIC ACIDS RESEARCH, 1988, 16 (10) :4525-4538

[3]

BENSASSON RV, 1983, FLASH PHOTOLYSIS PUL, P121

[4] INDUCTION OF POLYNUCLEOTIDE-PROTEIN CROSS-LINKAGES BY ULTRAVIOLET-IRRADIATION - PECULIARITIES OF THE HIGH-INTENSITY LASER-PULSE IRRADIATION [J].

BUDOWSKY, EI ;

AXENTYEVA, MS ;

ABDURASHIDOVA, GG ;

SIMUKOVA, NA ;

RUBIN, LB .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 159 (01) :95-101

[5]

CARERI G, 1975, Critical Reviews in Biochemistry, V3, P141, DOI 10.3109/10409237509102555

[6] THE ENHANCERS AND PROMOTERS OF THE XENOPUS-LAEVIS RIBOSOMAL SPACER ARE ASSOCIATED WITH HISTONES UPON ACTIVE TRANSCRIPTION OF THE RIBOSOMAL GENES [J].

DIMITROV, SI ;

STEFANOVSKY, VY ;

KARAGYOZOV, L ;

ANGELOV, D ;

PASHEV, IG .

NUCLEIC ACIDS RESEARCH, 1990, 18 (21) :6393-6397

[7]

DUBROV EN, 1989, PHOTOCHEM PHOTOBIOL, V49, P595

[8] LASER CROSSLINKING OF ESCHERICHIA-COLI RNA-POLYMERASE AND T7-DNA [J].

HARRISON, CA ;

TURNER, DH ;

HINKLE, DC .

NUCLEIC ACIDS RESEARCH, 1982, 10 (07) :2399-2413

[9]

HOCKENSMITH JW, 1986, J BIOL CHEM, V261, P2512

[10]

HOCKENSMITH JW, IN PRESS METHODS ENZ

← 1 2 →