ELECTRICAL MIGRATION IN CONVECTIVE ELECTROPHORESIS - MODELING AND MEMBRANE SEPARATION OF BOVUM SERUM-ALBUMIN (BSA) AND CYTOCHROME-C

被引：8

作者：

KONTTURI, AK ^{[1
]}

KONTTURI, K ^{[1
]}

SAVONEN, A ^{[1
]}

VUORISTO, M ^{[1
]}

SCHIFFRIN, DJ ^{[1
]}

机构：

[1] UNIV LIVERPOOL,DEPT CHEM,LIVERPOOL L69 3BX,ENGLAND

来源：

JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS | 1993年 / 89卷 / 01期

关键词：

D O I：

10.1039/ft9938900099

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The development of a continuous convective electrophoresis method for the separation of proteins is presented. The technique is based on opposing a convective flow across a porous membrane to electrical migration and diffusion. The Nernst-Planck equations describing protein transfer are solved and the effect of charge on the protein molecule is shown to be of great importance for achieving high selectivity ratios. From simulations of separation processes based on the solution of the migration, diffusion and convection problem, it is shown that the proposed technique can become a new powerful electro-assisted separation process. The experimental results were in good agreement with the predictions of the mathematical model.

引用

页码：99 / 106

页数：8

共 30 条

[11]

KONTTURI AK, 1987, ACTA POLYTECH SCAND, V178, P143

[12] TRANSPORT OF ACETATE AND CHLOROACETATE WEAK ELECTROLYTES THROUGH A THIN POROUS MEMBRANE IN COUNTERCURRENT ELECTROLYSIS [J].