ISOLATION OF MONOCLONAL-ANTIBODIES MONOSPECIFIC FOR BOVINE K-CASEIN

被引：15

作者：

KUZMANOFF, KM

ANDRESEN, JW

BEATTIE, CW

机构：

[1] Specialized Center for Cancer Research and Education, University of Illinois School of Medicine at Chicago, Chicago, Illinois, 60612

来源：

JOURNAL OF DAIRY SCIENCE | 1990年 / 73卷 / 10期

关键词：

milk proteins; monoclonal antibodies; κ-casein;

D O I：

10.3168/jds.S0022-0302(90)78959-X

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Bovine κ-casein represents a major portion of the total protein present in milk and is required for formation of the caseinate micelles responsible for the transportation of both calcium and phosphorous. Two monoclonal antibodies directed against bovine κ-casein have been isolated. Both monoclonal antibodies are highly specific for bovine κ-casein. Western analysis of denatured κ-casein suggests epitope specificity is, in part, conformationally dependent. Additional epitope mapping with chymosin and neuraminidase also suggest antibody binding is in the region of the amino acid sequence Pro-Thr-Thr at positions 92 to 94 and 134 to 136. © 1990, American Dairy Science Association. All rights reserved.

引用

页码：2741 / 2748

页数：8

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