CDNA AND DEDUCED AMINO-ACID-SEQUENCES OF A DOG LIVER CYTOCHROME-P-450 OF THE IIIA GENE SUBFAMILY

被引：45

作者：

CIACCIO, PJ

GRAVES, PE

BOURQUE, DP

GLINSMANNGIBSON, B

HALPERT, JR

机构：

[1] UNIV ARIZONA,DEPT BIOCHEM,TUCSON,AZ 85721

[2] UNIV ARIZONA,COLL PHARM,DEPT NEPHROL,TUCSON,AZ 85721

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1088卷 / 02期

关键词：

CYTOCHROME-P-450; P450IIIA; LIVER; CDNA SEQUENCE; (DOG);

D O I：

10.1016/0167-4781(91)90072-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 1.96 kbp cDNA encoding a male Beagle dog liver cytochrome P-450 of 503 amino acid residues (M(r) 57 636) has been isolated and sequenced. The deduced amino acid sequence is 79.8%, 69.3% and 74.1% identical to the P450IIIA forms human NF25, rat PCN1 and rabbit LM3c, respectively. The amino terminal sequence is identical to the first 28 residues of the dog P450IIIA form PBD-1. Southern blot analysis yields restriction patterns consistent with IIIA gene subfamily multiplicity.

引用

页码：319 / 322

页数：4

共 22 条

[21] HUMAN-LIVER MICROSOMAL STEROID-METABOLISM - IDENTIFICATION OF THE MAJOR MICROSOMAL STEROID-HORMONE 6-BETA-HYDROXYLASE CYTOCHROME-P-450 ENZYME [J].