INTEGRATED REACTION AND PHOTOMETRIC DETECTION WITH ENZYMES IMMOBILIZED IN THE FLOW CELL OF A FLOW-INJECTION SYSTEM

被引：25

作者：

LINARES, P ^{[1
]}

DECASTRO, L ^{[1
]}

VALCARCEL, M ^{[1
]}

机构：

[1] UNIV CORDOBA,FAC SCI,DEPT ANALYT CHEM,CORDOBA,SPAIN

来源：

ANALYTICA CHIMICA ACTA | 1990年 / 230卷 / 01期

关键词：

D O I：

10.1016/S0003-2670(00)82782-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A biosensor based on the integration of reaction and detection with a conventional photometric detector is proposed. An enzyme is immobilized on the walls of the flow cell and reaction-rate measurements of the enzymatic reaction are made by halting the flow when the sample-coenzyme plug is in the flow cell and monitoring the increase in the reduced form of the coenzyme (λmax = 340 nm). © 1990.

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页码：199 / 202

页数：4

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