GLYCOSIDASES IN ORGANIC-SOLVENTS .1. ALKYL-BETA-GLUCOSIDE SYNTHESIS IN A WATER-ORGANIC 2-PHASE SYSTEM

被引：83

作者：

VULFSON, EN

PATEL, R

BEECHER, JE

ANDREWS, AT

LAW, BA

机构：

[1] Department of Biotechnology and Enzymology, AFRC Institute of Food Research, Shinfield, Reading

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1990年 / 12卷 / 12期

关键词：

alkyl-β-glucoside synthesis; Water-organic two-phase system; β-glucosidase;

D O I：

10.1016/0141-0229(90)90115-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A low-water organic solvent two-phase system suitable for glycosylation of hydrophobic substrates is described. Almond β-glucosidase adsorbed on polymeric supports has been shown to catalyse alkyl-β-glucoside synthesis via a transferase reaction or through direct condensation of the glucosidic bond. High concentrations of glucosyl donors were present in the aqueous phase, while water-immiscible primary alcohols, which form the organic phase, served as acceptors of glucose. Reaction yield appeared to be thermodynamically controlled. The influence of various support materials, glucosyl donors, and glucosyl acceptors on reaction rate and product yield was investigated. © 1990.

引用

页码：950 / 954

页数：5

共 18 条

[1] REGIOSELECTIVE SYNTHESIS OF TRISACCHARIDES BY USE OF A REVERSED HYDROLYSIS ACTIVITY OF ALPHA-D-GALACTOSIDASE AND BETA-D-GALACTOSIDASE [J].