MOLECULAR-CLONING AND CHARACTERIZATION OF A MAJOR ALLERGEN (MYR PI) FROM THE VENOM OF THE AUSTRALIAN JUMPER ANT, MYRMECIA-PILOSULA

被引：49

作者：

DONOVAN, GR

BALDO, BA

SUTHERLAND, S

机构：

[1] ROYAL N SHORE HOSP,INST MED RES,ST LEONARDS,NSW 2065,AUSTRALIA

[2] UNIV SYDNEY,DEPT MED,SYDNEY,NSW 2006,AUSTRALIA

[3] COMMONWEALTH SERUM LABS,PARKVILLE,VIC 3052,AUSTRALIA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1171卷 / 03期

关键词：

MOLECULAR CLONING; CHARACTERIZATION; ALLERGEN; IGE; (MYRMECIA-PILOSULA);

D O I：

10.1016/0167-4781(93)90065-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Five IgE-binding components were identified in the venom of the Australian jumper ant, Myrmecia pilosula using SDS polyacrylamide gel electrophoresis and Western blotting. A cDNA clone which encodes the entire amino acid sequence of one of the major IgE-binding venom allergens has been nucleotide sequenced. The IgE-binding determinants of this allergen are located in its C-terminal domain. Database searches, however, did not reveal any homology with any other known nucleotide or protein sequence. The sequenced allergenic polypeptide has, according to the convention recommended by the International Union of Immunological Societies (IUIS), been named Myr p I.

引用

页码：272 / 280

页数：9