ISOLATION AND CHARACTERIZATION OF A NOVEL GLUCURONOSYL DIACYLGLYCEROL FROM MYCOBACTERIUM-SMEGMATIS

被引：25

作者：

WOLUCKA, BA ^{[1
]}

MCNEIL, MR ^{[1
]}

KALBE, L ^{[1
]}

COCITO, C ^{[1
]}

BRENNAN, PJ ^{[1
]}

机构：

[1] COLORADO STATE UNIV,DEPT MICROBIOL,FT COLLINS,CO 80523

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1170卷 / 02期

关键词：

GLYCOLIPID; MYCOBACTERIUM; GLUCURONIC ACID; ANTIGENIC MYCOBACTERIAL GLYCOLIPID; FAB-MS; NMR; H-1-(-);

D O I：

10.1016/0005-2760(93)90062-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A glucuronic acid-containing diacylglycerol was isolated from exponentially growing Mycobacterium smegmatis. Structural analysis of the purified glycolipid, performed by gas chromatography-mass spectrometry, fast atom bombardment-mass spectrometry, and high resolution proton NMR, indicated the structure 3-(O-alpha-D-glucuronopyranosyl)-1,2-diacyl-sn-glycerol. Two forms of the glycolipid were observed differing in fatty acid composition. Both molecular species contained a hexadecanoic acid residue, whereas the second acyl group was either tuberculostearic acid (10-methylstearic acid) or octadecenoic acid. The inherent antigenicity of the glycolipid was shown by its ability to bind to anti-Mycobacterium avium (serovar 26) and anti-Mycobacterium tuberculosis sera by Western blot-type thin-layer chromatography. This is the second instance of the isolation of a glycosyl diacylglycerol from members of the Mycobacterium genus, further confirming its close relationship to Gram-positive bacteria.

引用

页码：131 / 136

页数：6

共 47 条

[11]

CHATTERJEE D, 1988, J BIOL CHEM, V263, P4092

[12]

CHATTERJEE D, 1992, J BIOL CHEM, V267, P6228

[13]

CHATTERJEE D, 1987, J BIOL CHEM, V262, P3528

[14] HYDROLYTIC PROCEDURE FOR THE IDENTIFICATION AND ESTIMATION OF INDIVIDUAL PHOSPHOLIPIDS IN BIOLOGICAL SAMPLES [J].

DAWSON, RMC .

BIOCHEMICAL JOURNAL, 1960, 75 :45-53

[15] PHYSIOLOGY OF LIPOTEICHOIC ACIDS IN BACTERIA [J].

FISCHER, W .

ADVANCES IN MICROBIAL PHYSIOLOGY, 1988, 29 :233-302

[16]

FISCHER W, 1980, J BIOL CHEM, V255, P4557

[17]

GANFIELD MCW, 1980, J BIOL CHEM, V255, P5164

[18] DETERMINATION OF D AND L CONFIGURATION OF NEUTRAL MONOSACCHARIDES BY HIGH-RESOLUTION CAPILLARY GLC [J].

GERWIG, GJ ;

KAMERLING, JP ;

VLIEGENTHART, JFG .

CARBOHYDRATE RESEARCH, 1978, 62 (02) :349-357

[19]

Goren M.B., 1979, TUBERCULOSIS, P63

[20]

HAKOMORI SI, 1964, J BIOCHEM-TOKYO, V55, P205

← 1 2 3 4 5 →