A POSTTARGETING SIGNAL SEQUENCE RECOGNITION EVENT IN THE ENDOPLASMIC-RETICULUM MEMBRANE

被引：237

作者：

JUNGNICKEL, B ^{[1
]}

RAPOPORT, TA ^{[1
]}

机构：

[1] MAX DELBRUCK CTR MOLEC MED,D-13125 BERLIN,GERMANY

来源：

CELL | 1995年 / 82卷 / 02期

关键词：

D O I：

10.1016/0092-8674(95)90313-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have analyzed early phases of the cotranslational transport of the secretory protein preprolactin through the mammalian endoplasmic reticulum (ER) membrane. Following recognition of the signal sequence of the nascent polypeptide chain in the cytosol by the SRP, the chain is transferred into the membrane, where a second signal sequence recognition step takes place for which the presence in the lipid bilayer of the Sec61p complex is essential and sufficient. This step leads to a tight junction between the ribosome-nascent chain complex and the Sec61p complex, and to the productive insertion of the nascent chain into the translocation site, These results show that a translocation substrate is subjected to two recognition events before being allowed to cross the ER membrane.

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页码：261 / 270

页数：10

相关论文

共 30 条

[1] RIBOSOME-MEMBRANE INTERACTION - NONDESTRUCTIVE DISASSEMBLY OF RAT-LIVER ROUGH MICROSOMES INTO RIBOSOMAL AND MEMBRANOUS COMPONENTS [J].

ADELMAN, MR ;

SABATINI, DD ;

BLOBEL, G .

JOURNAL OF CELL BIOLOGY, 1973, 56 (01) :206-229

[2] INVIVO FUNCTION AND MEMBRANE-BINDING PROPERTIES ARE CORRELATED FOR ESCHERICHIA-COLI LAMB SIGNAL PEPTIDES [J].

BRIGGS, MS ;

GIERASCH, LM ;

ZLOTNICK, A ;

LEAR, JD ;

DEGRADO, WF .

SCIENCE, 1985, 228 (4703) :1096-1099

[3] ACCESS OF PROTEINASE-K TO PARTIALLY TRANSLOCATED NASCENT POLYPEPTIDES IN INTACT AND DETERGENT-SOLUBILIZED MEMBRANES [J].

CONNOLLY, T ;

COLLINS, P ;

GILMORE, R .

JOURNAL OF CELL BIOLOGY, 1989, 108 (02) :299-307

[4] THE SIGNAL RECOGNITION PARTICLE RECEPTOR MEDIATES THE GTP-DEPENDENT DISPLACEMENT OF SRP FROM THE SIGNAL SEQUENCE OF THE NASCENT POLYPEPTIDE [J].

CONNOLLY, T ;

GILMORE, R .

CELL, 1989, 57 (04) :599-610

[5] THE SIGNAL SEQUENCE MOVES THROUGH A RIBOSOMAL TUNNEL INTO A NONCYTOPLASMIC AQUEOUS ENVIRONMENT AT THE ER MEMBRANE EARLY IN TRANSLOCATION [J].

CROWLEY, KS ;

REINHART, GD ;

JOHNSON, AE .

CELL, 1993, 73 (06) :1101-1115

[6] SECRETORY PROTEINS MOVE THROUGH THE ENDOPLASMIC-RETICULUM MEMBRANE VIA AN AQUEOUS, GATED PORE [J].

CROWLEY, KS ;

LIAO, SR ;

WORRELL, VE ;

REINHART, GD ;

JOHNSON, AE .

CELL, 1994, 78 (03) :461-471

[7] SUPPRESSOR MUTATIONS THAT RESTORE EXPORT OF A PROTEIN WITH A DEFECTIVE SIGNAL SEQUENCE [J].

EMR, SD ;

HANLEYWAY, S ;

SILHAVY, TJ .

CELL, 1981, 23 (01) :79-88

[8] TRANSIENT INVOLVEMENT OF SIGNAL RECOGNITION PARTICLE AND ITS RECEPTOR IN THE MICROSOMAL MEMBRANE PRIOR TO PROTEIN TRANSLOCATION [J].

GILMORE, R ;

BLOBEL, G .

CELL, 1983, 35 (03) :677-685

[9]

GILMORE R, 1992, J CELL BIOL, V95, P470

[10] A PROTEIN OF THE ENDOPLASMIC-RETICULUM INVOLVED EARLY IN POLYPEPTIDE TRANSLOCATION [J].

GORLICH, D ;

HARTMANN, E ;

PREHN, S ;

RAPOPORT, TA .

NATURE, 1992, 357 (6373) :47-52