MITOTIC REGULATOR PROTEIN RCC1 IS COMPLEXED WITH A NUCLEAR RAS-RELATED POLYPEPTIDE

被引：245

作者：

BISCHOFF, FR ^{[1
]}

PONSTINGL, H ^{[1
]}

机构：

[1] GERMAN CANC RES CTR,MOLEC BIOL MITOSIS PROJECT,NEUENHEIMER FELD 280,W-6900 HEIDELBERG,GERMANY

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 23期

关键词：

RAN PROTEIN; AMINO ACID SEQUENCE; GDP GTP-BINDING PROTEIN; MATURATION-PROMOTING FACTOR;

D O I：

10.1073/pnas.88.23.10830

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We previously reported the purification of a complex of two proteins from human chromatin, consisting of a 47-kDa component called RCC1, which is a negative regulator of mitosis, and a 25-kDa protein. Here we show that the 25-kDa protein has a ras-related sequence. It binds guanine nucleotides, and excess Mg2+ and GDP or GTP dissociate the complex. Immunofluorescence studies and biochemical properties indicate that this polypeptide, in contrast to most members of the Ras family, is present in the nucleoplasm as a soluble monomer, in 25-fold excess over the complexed form. We designate this polypeptide Ran, for ras-related nuclear protein.

引用

页码：10830 / 10834

页数：5

共 14 条

[1] A 47-KDA HUMAN NUCLEAR-PROTEIN RECOGNIZED BY ANTIKINETOCHORE AUTOIMMUNE SERA IS HOMOLOGOUS WITH THE PROTEIN ENCODED BY RCC1, A GENE IMPLICATED IN ONSET OF CHROMOSOME CONDENSATION [J].

BISCHOFF, FR ;

MAIER, G ;

TILZ, G ;

PONSTINGL, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (21) :8617-8621

[2]

BISCHOFF FR, 1991, IN PRESS NATURE LOND

[3] CELL-CYCLE CONTROL IN EUKARYOTES - MOLECULAR MECHANISMS OF CDC2 ACTIVATION [J].

DRAETTA, G .

TRENDS IN BIOCHEMICAL SCIENCES, 1990, 15 (10) :378-383

[4] CHARACTERIZATION OF 4 NOVEL RAS-LIKE GENES EXPRESSED IN A HUMAN TERATOCARCINOMA CELL-LINE [J].

DRIVAS, GT ;

SHIH, A ;

COUTAVAS, E ;

RUSH, MG ;

DEUSTACHIO, P .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (04) :1793-1798

[5]

JOHN J, 1988, J BIOL CHEM, V263, P11792

[6] MOLECULAR-CLONING OF A HUMAN-GENE THAT REGULATES CHROMOSOME CONDENSATION AND IS ESSENTIAL FOR CELL-PROLIFERATION [J].

KAI, R ;

OHTSUBO, M ;

SEKIGUCHI, M ;

NISHIMOTO, T .

MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (06) :2027-2032

[7] PREMATURE INITIATION OF MITOSIS IN YEAST LACKING RCC1 OR AN INTERACTING GTPASE [J].

MATSUMOTO, T ;

BEACH, D .

CELL, 1991, 66 (02) :347-360

[8] THE SYNTHESIS OF PROTEIN(S) FOR CHROMOSOME CONDENSATION MAY BE REGULATED BY A POST-TRANSCRIPTIONAL MECHANISM [J].

NISHIMOTO, T ;

ISHIDA, R ;

AJIRO, K ;

YAMAMOTO, S ;

TAKAHASHI, T .

JOURNAL OF CELLULAR PHYSIOLOGY, 1981, 109 (02) :299-308

[9] PREMATURE CHROMOSOME CONDENSATION IN A TS DNA-MUTANT OF BHK CELLS [J].

NISHIMOTO, T ;

EILEN, E ;

BASILICO, C .

CELL, 1978, 15 (02) :475-483

[10] LOSS OF RCC1, A NUCLEAR DNA-BINDING PROTEIN, UNCOUPLES THE COMPLETION OF DNA-REPLICATION FROM THE ACTIVATION OF CDC2 PROTEIN-KINASE AND MITOSIS [J].

NISHITANI, H ;

OHTSUBO, M ;

YAMASHITA, K ;

IIDA, H ;

PINES, J ;

YASUDO, H ;

SHIBATA, Y ;

HUNTER, T ;

NISHIMOTO, T .

EMBO JOURNAL, 1991, 10 (06) :1555-1564

← 1 2 →