STUDIES OF NUCLEAR RESIDUAL PROTEINS

被引：28

作者：

PATEL, G

PATEL, V

WANG, TY

ZOBEL, CR

机构：

[1] Department of Zoology, University of Georgia, Athens

[2] Department of Biology and Biophysics, State University of New York, Buffalo

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1968年 / 128卷 / 03期

关键词：

D O I：

10.1016/0003-9861(68)90075-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nuclear residual proteins of rat liver have been prepared by solubilization with sodium deoxycholate and Sephadex gel filtration. These proteins were examined by analytical ultracentrifugation, DEAE-cellulose chromatography, starch gel electrophoresis, amino acid composition, and alkali-labile phosphorus analysis. The results show that these proteins are heterogeneous, having a high glutamic acid content, and contain phosphoprotein. Partial fractionation was achieved by DEAE-cellulose chromatography and starch gel electrophoresis. Some of these proteins appeared as spheroidal particles with diameters of 100-400 Å. In the presence of sodium deoxycholate, the particles dissociated into smaller units that reassociated upon removal of the detergent. © 1968.

引用

页码：654 / &