NEAR-FIELD FLUORESCENT IMAGING OF SINGLE PROTEINS

被引：36

作者：

DUNN, RC ^{[1
]}

ALLEN, EV ^{[1
]}

JOYCE, SA ^{[1
]}

ANDERSON, GA ^{[1
]}

XIE, XS ^{[1
]}

机构：

[1] PACIFIC NW LAB, MOLEC SCI RES CTR, RICHLAND, WA 99352 USA

来源：

ULTRAMICROSCOPY | 1995年 / 57卷 / 2-3期

关键词：

D O I：

10.1016/0304-3991(94)00121-3

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

We report the results of our initial efforts to image photosynthetic proteins using the near-field technique. Allophycocyanin trimers, which contains six chromophores each, were dispersed onto a glass cover slip, and the fluorescence was imaged using a recently constructed near-field microscope. The results show that the emission from single proteins can be detected and localized on the nanometer scale.

引用

页码：113 / 117

页数：5

共 12 条

[1] NEAR-FIELD OPTICS - MICROSCOPY, SPECTROSCOPY, AND SURFACE MODIFICATION BEYOND THE DIFFRACTION LIMIT [J].

BETZIG, E ;

TRAUTMAN, JK .

SCIENCE, 1992, 257 (5067) :189-195

[2] COMBINED SHEAR FORCE AND NEAR-FIELD SCANNING OPTICAL MICROSCOPY [J].

BETZIG, E ;

FINN, PL ;

WEINER, JS .

APPLIED PHYSICS LETTERS, 1992, 60 (20) :2484-2486

[3] BREAKING THE DIFFRACTION BARRIER - OPTICAL MICROSCOPY ON A NANOMETRIC SCALE [J].

BETZIG, E ;

TRAUTMAN, JK ;

HARRIS, TD ;

WEINER, JS ;

KOSTELAK, RL .

SCIENCE, 1991, 251 (5000) :1468-1470

[4] SINGLE MOLECULES OBSERVED BY NEAR-FIELD SCANNING OPTICAL MICROSCOPY [J].

BETZIG, E ;

CHICHESTER, RJ .

SCIENCE, 1993, 262 (5138) :1422-1425

[5]

BRYANT DA, 1976, ARCH MICROBIOL, V110, P60

[6] ISOLATION, CRYSTALLIZATION, CRYSTAL-STRUCTURE ANALYSIS AND REFINEMENT OF CONSTITUTIVE C-PHYCOCYANIN FROM THE CHROMATICALLY ADAPTING CYANOBACTERIUM FREMYELLA-DIPLOSIPHON AT 1.66 A RESOLUTION [J].

DUERRING, M ;

SCHMIDT, GB ;

HUBER, R .

JOURNAL OF MOLECULAR BIOLOGY, 1991, 217 (03) :577-592

[7]

Guard-Friar D., 1987, PHYCOBILIPROTEINS

[8] BILIPROTEIN ASSEMBLY IN THE DISC-SHAPED PHYCOBILISOMES OF RHODELLA-VIOLACEA ELECTRON MICROSCOPICAL AND BIOCHEMICAL ANALYSES - OF C-PHYCOCYANIN AND ALLOPHYCOCYANIN AGGREGATES [J].

MORSCHEL, E ;

KOLLER, KP ;

WEHRMEYER, W .

ARCHIVES OF MICROBIOLOGY, 1980, 125 (1-2) :43-51

[9]

ONG LJ, 1985, PHYSIOL VEG, V23, P777

[10]

SCHEER H, COMMUNICATION

← 1 2 →