IMPROVED DETECTION LIMITS FOR SCREENING OF DIURETICS BY COUPLED LIQUID-CHROMATOGRAPHY AND ULTRAVIOLET VISIBLE SPECTROPHOTOMETRY

被引：19

作者：

CAMPINSFALCO, P

HERRAEZHERNANDEZ, R

SEVILLANOCABEZ, A

机构：

[1] Departamento de Química Analítica, Facultad de Química, Universidad de Valencia, Burjassot, Valencia

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 612卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)80170-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Experimental conditions have been studied in order to improve the sensitivity for the analysis of diuretics and probenecid in urine samples by high-performance liquid chromatography with ultraviolet detection. Sample clean-up and chromatographic parameters have been optimized to obtain a suitable sensitivity for the detection or quantification of each diuretic using an HP-Hypersil ODS-C18 column (5 mum, 250 mm x 4 mm I.D.), taking into account the pharmacological properties of each compound. The reliability of this method was tested by analysing urine samples after a minimum single-dose administration of chlorthalidone and probenecid.

引用

页码：245 / 251

页数：7

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