PHOSPHORYLATION OF POLY(ADP-RIBOSE)POLYMERASE PROTEIN IN HUMAN PERIPHERAL LYMPHOCYTES STIMULATED WITH PHYTOHEMAGGLUTININ

被引：12

作者：

BAUER, PI

FARKAS, G

MIHALIK, R

KOPPER, L

KUN, E

FARAGO, A

机构：

[1] SEMMELWEIS UNIV MED, SCH MED, DEPT BIOCHEM 1, H-1444 BUDAPEST 8, HUNGARY

[2] SEMMELWEIS UNIV MED, SCH MED, DEPT BIOCHEM 2, H-1444 BUDAPEST 8, HUNGARY

[3] NATL INST HAEMATOL & BLOOD TRANSFUS, H-1113 BUDAPEST, HUNGARY

[4] SEMMELWEIS UNIV MED, SCH MED, INST PATHOL & EXPTL CANC RES, H-1085 BUDAPEST, HUNGARY

[5] SAN FRANCISCO STATE UNIV, ROMBERG TIBURON CTR, ENVIRONM TOXICOL & CHEM LAB, TIBURON, CA 94920 USA

[6] SAN FRANCISCO STATE UNIV, ROMBERG TIBURON CTR, OCTAMER RES FDN, TIBURON, CA 94920 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1994年 / 1223卷 / 02期

基金：

匈牙利科学研究基金会;

关键词：

POLY(ADP-RIBOSE)POLYMERASE; PROTEIN KINASE; PHORBOL MYRISTYL ACETATE; PHYTOHEMAGGLUTININ; SIGNAL TRANSDUCTION; PHOSPHORYLATION; (LYMPHOCYTE);

D O I：

10.1016/0167-4889(94)90231-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Intracellular phosphorylation of poly(ADP-ribose)polymerase was assayed in streptolysin-O-permeabilized human lymphocytes. Whereas P-32 incorporation from [gamma-P-32]ATP into immunoprecipitated enzyme protein was undetectable in resting cells, significant phosphorylation of this enzyme was observed in lymphocytes treated with phytohemagglutinin. The phosphorylation of poly(ADP-ribose)polymerase in permeabilized cells was not stimulated by phorbol ester, while phorbol-induced phosphorylation of other proteins and of a specific oligopeptide substrate of protein kinase C was observed. However, the specific inhibitory pseudosubstrate peptide of protein kinase C blocked the phosphorylation of poly(PLDP-ribose)polymerase induced by phytohemagglutinin. Therefore, a potential role of a member of the protein kinase C family in the phytohemagglutinin stimulated intracellular phosphorylation of poly(ADP-ribose)polymerase is conceivable.

引用

页码：234 / 239

页数：6

共 23 条

[1] A PROTEIN KINASE-C PSEUDOSUBSTRATE PEPTIDE INHIBITS PHOSPHORYLATION OF THE CD3 ANTIGEN IN STREPTOLYSIN-O-PERMEABILIZED HUMAN LYMPHOCYTES-T
ALEXANDER, DR
HEXHAM, JM
LUCAS, SC
GRAVES, JD
CANTRELL, DA
CRUMPTON, MJ
[J]. BIOCHEMICAL JOURNAL, 1989, 260 (03) : 893 - 901
[2] INHIBITION OF DNA-BINDING BY THE PHOSPHORYLATION OF POLY ADP-RIBOSE POLYMERASE PROTEIN CATALYZED BY PROTEIN-KINASE-C
BAUER, PI
FARKAS, G
BUDAY, L
MIKALA, G
MESZAROS, G
KUN, E
FARAGO, A
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 187 (02) : 730 - 736
[3] EVIDENCE FOR THE PARTICIPATION OF HISTIDINE-RESIDUES LOCATED IN THE 56 KDA C-TERMINAL POLYPEPTIDE DOMAIN OF ADP-RIBOSYL TRANSFERASE IN ITS CATALYTIC ACTIVITY
BAUER, PI
BUKI, KG
KUN, E
[J]. FEBS LETTERS, 1990, 273 (1-2) : 6 - 10
[4] PROTEIN KINASE-C AND T-CELL ACTIVATION
BERRY, N
NISHIZUKA, Y
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 189 (02): : 205 - 214
[5] BOYUM A, 1968, SCAND J CLIN LAB INV, VS 21, P31
[6] DUAL EFFECT OF ARACHIDONIC-ACID ON PROTEIN-KINASE-C ISOENZYMES ISOLATED FROM RABBIT THYMUS-CELLS
BUDAY, L
FARAGO, A
[J]. FEBS LETTERS, 1990, 276 (1-2) : 223 - 226
[7] PROTEOLYTIC ACTIVATION OF PROTEIN-KINASE-C IN THE EXTRACTS OF CELLS TREATED FOR A SHORT-TIME WITH PHORBOL ESTER
BUDAY, L
SEPRODI, J
FARKAS, G
MESZAROS, G
ROMHANYI, T
BANHEGYI, G
MANDL, J
ANTONI, F
FARAGO, A
[J]. FEBS LETTERS, 1987, 223 (01) : 15 - 19
[8] ISOLATION OF ADENOSINE DIPHOSPHORIBOSYLTRANSFERASE BY PRECIPITATION WITH REACTIVE RED-120 COMBINED WITH AFFINITY-CHROMATOGRAPHY
BUKI, KG
KIRSTEN, E
KUN, E
[J]. ANALYTICAL BIOCHEMISTRY, 1987, 167 (01) : 160 - 166
[9] PROTEIN-KINASE-C IN TRANSMEMBRANE SIGNALING
FARAGO, A
NISHIZUKA, Y
[J]. FEBS LETTERS, 1990, 268 (02) : 350 - 354
[10] PROTEIN-KINASE-C CONTAINS A PSEUDOSUBSTRATE PROTOTYPE IN ITS REGULATORY DOMAIN
HOUSE, C
KEMP, BE
[J]. SCIENCE, 1987, 238 (4834) : 1726 - 1728

← 1 2 3 →