INSITU CHARACTERIZATION OF TISSUE LYMPHOID-CELLS OF CUTANEOUS INFILTRATES USING SPECIFIC MEMBRANE-ANTIGENS

被引：35

作者：

SCHMITT, D

ALARIO, A

THIVOLET, J

机构：

[1] Recherche Dermatologique et Immunologie, Clinique Dermatologique, Hôpital Edouard Herriot, Lyon

来源：

CLINICAL AND EXPERIMENTAL DERMATOLOGY | 1979年 / 4卷 / 02期

关键词：

D O I：

10.1111/j.1365-2230.1979.tb01614.x

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

A method for identification of B and T lymphocytes on frozen tissue sections is described. The technique was initially established on mouse spleen. Thymodependant cells were identified by anti‐Thy‐i and anti‐theta (alloantigens specific for mouse T lymphocytes) sera and thymoindependant cells by Fab‐Po anti‐Ig serum. This technique was subsequently applied to a study of benign and malignant cutaneous lymphoid infiltrates. Specific membrane markers, human B‐lymphocyte antigens (HBLA) for B lymphocytes and human T‐lymphocyte antigens (HTLA) for T lymphocytes, were revealed by peroxidase immunolabelling. A prominent T‐Iymphocyte population was observed in the cutaneous lesions of sarcoidosis, lichen planus, halo naevus and lupus erythematosus. In contact dermatitis, the prominence of the T‐cell infiltrate is in accordance with the delayed hypersensitivity reaction. Immunoenzymatic labelling of sections of epidermotropic malignant cutaneous lymphoma confirmed the T‐cell nature of the intraepidermal neoplastic cells. This technique of direct specific labelling on sections should also be applicable to electron microscopy. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：161 / 173

页数：13

共 30 条

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