INSITU CHARACTERIZATION OF TISSUE LYMPHOID-CELLS OF CUTANEOUS INFILTRATES USING SPECIFIC MEMBRANE-ANTIGENS

被引：35

作者：

SCHMITT, D

ALARIO, A

THIVOLET, J

机构：

[1] Recherche Dermatologique et Immunologie, Clinique Dermatologique, Hôpital Edouard Herriot, Lyon

来源：

CLINICAL AND EXPERIMENTAL DERMATOLOGY | 1979年 / 4卷 / 02期

关键词：

D O I：

10.1111/j.1365-2230.1979.tb01614.x

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

A method for identification of B and T lymphocytes on frozen tissue sections is described. The technique was initially established on mouse spleen. Thymodependant cells were identified by anti‐Thy‐i and anti‐theta (alloantigens specific for mouse T lymphocytes) sera and thymoindependant cells by Fab‐Po anti‐Ig serum. This technique was subsequently applied to a study of benign and malignant cutaneous lymphoid infiltrates. Specific membrane markers, human B‐lymphocyte antigens (HBLA) for B lymphocytes and human T‐lymphocyte antigens (HTLA) for T lymphocytes, were revealed by peroxidase immunolabelling. A prominent T‐Iymphocyte population was observed in the cutaneous lesions of sarcoidosis, lichen planus, halo naevus and lupus erythematosus. In contact dermatitis, the prominence of the T‐cell infiltrate is in accordance with the delayed hypersensitivity reaction. Immunoenzymatic labelling of sections of epidermotropic malignant cutaneous lymphoma confirmed the T‐cell nature of the intraepidermal neoplastic cells. This technique of direct specific labelling on sections should also be applicable to electron microscopy. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：161 / 173

页数：13

共 30 条

[21]

Schmitt D., Alario A., Touraine J.L., Claudy A.L., Perrot H., Thivolet J., Specific surface antigen of human B and T lymphocytes: the use in the ultrastructural identification of cells extracted from cutaneous infiltrations. Fourth European Meeting of Electron Microscopy applied to Cutaneous Pathology, 6–7 May 1977, Journal of Cutaneous Pathology, 4, (1977)

[22]

Schmitt D., Viac J., Brochier J., Tmvolet J., Thymus derived origin of Sezary cells demonstrated by pcroxidase conjugated anti HTLA serum, Acta dermato-venereologica, 56, pp. 489-491, (1976)

[23]

Schoorl R., Brutel de la Riviere A., von dem Borne, Feltkamp Vroom T.M., Identification of T and B lymphocytes in human breast cancer with immunohistochemical techniques, American Journal of Pathology, 84, pp. 529-540, (1976)

[24]

Smith R.W., Terry W.D., Buell D.N., Sell K.W., An antigenic marker for human thymic lymphocytes, Journal of Immunology, 110, pp. 884-889, (1973)

[25]

Stimgl G., Wolff K., Diem E., Baumgartner G., Knapp W., In situ Identification of lymphoreticular cells in benign and malignant infiltrates by membrane receptor sites, Journal of Investigative Dermatology, 69, pp. 231-235, (1977)

[26]

Tan R.S., Byron M.A., Hayes J.P., A method of liberating living cells from the dermal infiltrate. Studies on skin reticulosis and lichen planus, British Journal of Dermatology, 93, pp. 271-276, (1975)

[27]

Touraine J.L., Touraine F.D., Bouteiller O., Rommeau M., Identification et enumeration des lymphocytes T et B chez l'homme en pratique quotidienne, Techniques de Separation ct d'identification des lymphocytes hutnains, 57, pp. 121-135, (1976)

[28]

Touraine J.L., Touraine F., Kiszkiss D.T., Choi Y.S., Good R.A., Heterologous specific antiserum for identification of human T lymphocytes, Clinical and Experimental Immunology, 16, pp. 503-520, (1974)

[29]

Walkee D.M., Identification of subpopulations of lymphocytes and macrophages in the infiltrates of lichen planus lesions of skin and oral mucosa, British Journal of Dermatology, 94, pp. 529-534, (1976)

[30]

Wybran J., Carr M.C., Fudenberg H.H., The human rosettes forming cells as a marker of a population of thymus–derived cells, Journal of Clinical Investigations, 51, pp. 2537-2543, (1972)

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