SEQUENCE OF A CLUSTER OF GENES-CONTROLLING SYNTHESIS AND SECRETION OF ALKALINE PROTEASE IN PSEUDOMONAS-AERUGINOSA - RELATIONSHIPS TO OTHER SECRETORY PATHWAYS

被引：145

作者：

DUONG, F ^{[1
]}

LAZDUNSKI, A ^{[1
]}

CAMI, B ^{[1
]}

MURGIER, M ^{[1
]}

机构：

[1] CNRS,CHIM BACTERIENNE LAB,31 CHEMIN JOSEPH AIGUIER,F-13277 MARSEILLE 9,FRANCE

来源：

GENE | 1992年 / 121卷 / 01期

关键词：

GRAM-NEGATIVE BACTERIA; METALLOPROTEASE; MULTIDRUG RESISTANCE-LIKE EXPORT SYSTEM; C-TERMINAL SECRETION SIGNAL; MEMBRANE TRANSLOCATORS; SECRETION FUNCTIONS; PROTEASE INHIBITOR;

D O I：

10.1016/0378-1119(92)90160-Q

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A genetic locus implicated in the synthesis and secretion of alkaline protease (APR) in Pseudomonas aeruginosa has been previously described [Guzzo et al., J. Bacteriol. 172 (1990) 942-948]. The nucleotide sequence of the DNA fragment encoding these functions was determined and revealed the existence of five open reading frames: aprA, the structural gene encoding APR; aprI, which encodes a protease inhibitor; and aprD, aprE, aprF whose products are involved in protease secretion. The AprD, AprE and AprF proteins share significant homology with proteins implicated in secretion of Erwinia chrysanthemi proteases and Escherichia coli alpha-haemolysin. These results provide further evidence for the existence of a specialized secretory stem widespread among Gram- bacteria.

引用

页码：47 / 54

页数：8

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