SEQUENCE OF A CLUSTER OF GENES-CONTROLLING SYNTHESIS AND SECRETION OF ALKALINE PROTEASE IN PSEUDOMONAS-AERUGINOSA - RELATIONSHIPS TO OTHER SECRETORY PATHWAYS

被引：145

作者：

DUONG, F ^{[1
]}

LAZDUNSKI, A ^{[1
]}

CAMI, B ^{[1
]}

MURGIER, M ^{[1
]}

机构：

[1] CNRS,CHIM BACTERIENNE LAB,31 CHEMIN JOSEPH AIGUIER,F-13277 MARSEILLE 9,FRANCE

来源：

GENE | 1992年 / 121卷 / 01期

关键词：

GRAM-NEGATIVE BACTERIA; METALLOPROTEASE; MULTIDRUG RESISTANCE-LIKE EXPORT SYSTEM; C-TERMINAL SECRETION SIGNAL; MEMBRANE TRANSLOCATORS; SECRETION FUNCTIONS; PROTEASE INHIBITOR;

D O I：

10.1016/0378-1119(92)90160-Q

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A genetic locus implicated in the synthesis and secretion of alkaline protease (APR) in Pseudomonas aeruginosa has been previously described [Guzzo et al., J. Bacteriol. 172 (1990) 942-948]. The nucleotide sequence of the DNA fragment encoding these functions was determined and revealed the existence of five open reading frames: aprA, the structural gene encoding APR; aprI, which encodes a protease inhibitor; and aprD, aprE, aprF whose products are involved in protease secretion. The AprD, AprE and AprF proteins share significant homology with proteins implicated in secretion of Erwinia chrysanthemi proteases and Escherichia coli alpha-haemolysin. These results provide further evidence for the existence of a specialized secretory stem widespread among Gram- bacteria.

引用

页码：47 / 54

页数：8

共 37 条

[11] CLONING OF XCP-GENES LOCATED AT THE 55-MIN REGION OF THE CHROMOSOME AND INVOLVED IN PROTEIN SECRETION IN PSEUDOMONAS-AERUGINOSA [J].

FILLOUX, A ;

BALLY, M ;

MURGIER, M ;

WRETLIND, B ;

LAZDUNSKI, A .

MOLECULAR MICROBIOLOGY, 1989, 3 (02) :261-265

[12] CLONING OF THE PSEUDOMONAS-AERUGINOSA ALKALINE PROTEASE GENE AND SECRETION OF THE PROTEASE INTO THE MEDIUM BY ESCHERICHIA-COLI [J].

GUZZO, J ;

MURGIER, M ;

FILLOUX, A ;

LAZDUNSKI, A .

JOURNAL OF BACTERIOLOGY, 1990, 172 (02) :942-948

[13] THE SECRETION GENES OF PSEUDOMONAS-AERUGINOSA ALKALINE PROTEASE ARE FUNCTIONALLY RELATED TO THOSE OF ERWINIA-CHRYSANTHEMI PROTEASES AND ESCHERICHIA-COLI ALPHA-HEMOLYSIN [J].

GUZZO, J ;

DUONG, F ;

WANDERSMAN, C ;

MURGIER, M ;

LAZDUNSKI, A .

MOLECULAR MICROBIOLOGY, 1991, 5 (02) :447-453

[14] PSEUDOMONAS-AERUGINOSA ALKALINE PROTEASE - EVIDENCE FOR SECRETION GENES AND STUDY OF SECRETION MECHANISM [J].

GUZZO, J ;

PAGES, JM ;

DUONG, F ;

LAZDUNSKI, A ;

MURGIER, M .

JOURNAL OF BACTERIOLOGY, 1991, 173 (17) :5290-5297

[15] ISOLATION OF THE ACTINOBACILLUS-PLEUROPNEUMONIAE HEMOLYSIN GENE AND THE ACTIVATION AND SECRETION OF THE PROHAEMOLYSIN BY THE HLYC, HLYB AND HLYD PROTEINS OF ESCHERICHIA-COLI [J].

GYGI, D ;

NICOLET, J ;

FREY, J ;

CROSS, M ;

KORONAKIS, V ;

HUGHES, C .

MOLECULAR MICROBIOLOGY, 1990, 4 (01) :123-128

[16] TRANSLOCATION AND ASSEMBLY OF OUTER-MEMBRANE PROTEINS OF ESCHERICHIA-COLI - SELECTIVE ACCUMULATION OF PRECURSORS AND NOVEL ASSEMBLY INTERMEDIATES CAUSED BY PHENETHYL ALCOHOL [J].

HALEGOUA, S ;

INOUYE, M .

JOURNAL OF MOLECULAR BIOLOGY, 1979, 130 (01) :39-61

[17] NUCLEOTIDE-SEQUENCE OF A PLASMID-ENCODED HEMOLYSIN DETERMINANT AND ITS COMPARISON WITH A CORRESPONDING CHROMOSOMAL HEMOLYSIN SEQUENCE [J].

HESS, J ;

WELS, W ;

VOGEL, M ;

GOEBEL, W .

FEMS MICROBIOLOGY LETTERS, 1986, 34 (01) :1-11

[18] A FAMILY OF RELATED ATP-BINDING SUBUNITS COUPLED TO MANY DISTINCT BIOLOGICAL PROCESSES IN BACTERIA [J].

HIGGINS, CF ;

HILES, ID ;

SALMOND, GPC ;

GILL, DR ;

DOWNIE, JA ;

EVANS, IJ ;

HOLLAND, IB ;

GRAY, L ;

BUCKEL, SD ;

BELL, AW ;

HERMODSON, MA .

NATURE, 1986, 323 (6087) :448-450

[19] CLUSTAL - A PACKAGE FOR PERFORMING MULTIPLE SEQUENCE ALIGNMENT ON A MICROCOMPUTER [J].

HIGGINS, DG ;

SHARP, PM .

GENE, 1988, 73 (01) :237-244

[20] THE SECRETED HEMOLYSINS OF PROTEUS-MIRABILIS, PROTEUS-VULGARIS, AND MORGANELLA-MORGANII ARE GENETICALLY RELATED TO EACH OTHER AND TO THE ALPHA-HEMOLYSIN OF ESCHERICHIA-COLI [J].

KORONAKIS, V ;

CROSS, M ;

SENIOR, B ;

KORONAKIS, E ;

HUGHES, C .

JOURNAL OF BACTERIOLOGY, 1987, 169 (04) :1509-1515

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