ELECTROPHORETICAL DIFFERENTIATION AND CLASSIFICATION OF PROTEINS .10. COMPARATIVE INVESTIGATION OF YEAST PROTEINS OF VARIOUS GENERA BY MEANS OF ISOELECTRIC-FOCUSING IN POLYACRYLAMIDE GELS

被引：7

作者：

DRAWERT, F

BEDNAR, J

机构：

[1] Institute of Food Technology and Analytical Chemistry, Technical University of Munich

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1979年 / 27卷 / 01期

关键词：

D O I：

10.1021/jf60221a021

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The water-soluble proteins of eight different yeast genera (Brettanomyces claussenii, Candida utilis, Cryptococcus laurentii, Debaryomyces hansenii, Kloeckera apiculata, Kluyveromyces lactis, Saccharomyces cerevisiae, Schizosaccharomyces pombe) obtained by cell disruption with freeze-pressing were resolved by means of isoelectric focusing in cylindrical polyacrylamide gels. In the pH range of 3.5-10.0 carrier ampholytes, about 30 protein bands were found with isoelectric points of 4.5 to 9.7. By using a special one-rod electrode, the pH of the gel slices in minute quantities of distilled water were measured, and the trend of the pH gradient in the whole gel was determined. By means of densitometer tracing of the protein pattern in connection with the pH gradient, the isoelectric points of particular proteins were established. The protein patterns were dissimilar and reproducible under constant working conditions. © 1979, American Chemical Society. All rights reserved.

引用

页码：3 / 6

页数：4

共 50 条

[1] CHARACTERIZATION AND COMPARISON OF MYCOBACTERIAL ANTIGENS BY DISCONTINUOUS PORE GRADIENT GEL-ELECTROPHORESIS [J].

AFFRONTI, LF ;

WRIGHT, GL ;

REICH, M .

INFECTION AND IMMUNITY, 1972, 5 (04) :474-+

[2]

ALLEN RC, 1974, ELECTROPHORESIS ISOE

[3]

AMES GFL, 1974, J BIOL CHEM, V249, P634

[4]

ARBUTHNOTT JP, 1975, ISOELECTRIC FOCUSING, P212

[5]

BARTON H, 1973, THESIS TU MUNCHEN WE

[6]

DONHAUSER S, 1976, BRAUWISSENSCHAFT, V29, P306

[7] DIFFERENTIATION AND CLASSIFICATION OF PROTEINS BY ELECTROPHORESIS .2. THIN-LAYER ISOELECTRIC FOCUSING OF PROTEINS FROM DIFFERENT GRAPE-VARIETIES [J].

DRAWERT, F ;

MULLER, W .

ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG, 1973, 153 (04) :204-212

[8] ELECTROPHORETICAL DIFFERENTIATION AND CLASSIFICATION OF PROTEINS .3. DISCELECTROPHORETICAL AND ISO-ELECTRICAL FOCUSING OF PROTEINS AND ENZYMES OF DIFFERENT GRAPE VARIETIES [J].

DRAWERT, F ;

GORG, A .

ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG, 1974, 154 (06) :328-338

[9]

Drawert F., 1974, Brauwissenschaft, V27, P89

[10]

Drawert F., 1978, Chemie Mikrobiologie Technologie der Lebensmittel, V5, P183

← 1 2 3 4 5 →