ROLES OF PYROPHOSPHATE - D-FRUCTOSE-6-PHOSPHATE 1-PHOSPHOTRANSFERASE AND FRUCTOSE-2,6-BISPHOSPHATE IN THE REGULATION OF GLYCOLYSIS DURING ACCLIMATION OF AEROBIC EUGLENA-GRACILIS TO ANAEROBIOSIS

被引：13

作者：

ENOMOTO, T

OHYAMA, H

INUI, H

MIYATAKE, K

NAKANO, Y

KITAOKA, S

机构：

[1] NARABUNKA WOMENS JR COLL,DEPT FOOD & NUTR,NARA 635,JAPAN

[2] UNIV OSAKA PREFECTURE,DEPT AGR CHEM,SAKAI,OSAKA 591,JAPAN

来源：

PLANT SCIENCE | 1990年 / 67卷 / 02期

关键词：

anaerobiosis; d-fructose-6-phosphate; 1-phosphotransferase; Euglena gracilis; fructose-2,6-bisphosphate; glycolysis; pyrophosphate;

D O I：

10.1016/0168-9452(90)90239-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

After aerobically grown Euglena gracilis was transferred to anaerobic conditions, the ATP level in the cells immediately decreased. Then, when O2 concentration in the medium decreased to less than 1 μM, wax ester fermentation, the anaerobic energy-generating system, operated and the ATP level was restored. The cellular level of fructose-2,6-bisphosphate (Fru-2,6-P2) also decreased immediately after the transfer, and markedly increased corresponding to the onset of fermentation. The activity of pyrophosphate: d-fructose-6-phosphate 1-phosphotransferase (PPi-PFK) changed depending on the Fru-2,6-P2 level. The activity in vivo of PPi-PFK was much higher than the metabolic rate of glycolysis during wax ester fermentation. From the results, the physiological function of PPi-PFK in E. gracilis is discussed. © 1990.

引用

页码：161 / 167

页数：7

共 30 条

[1] COMPARISON OF ACTIVITIES AND PROPERTIES OF PYROPHOSPHATE-DEPENDENT AND ADENOSINE TRIPHOSPHATE-DEPENDENT PHOSPHOFRUCTOKINASES OF BLACK GRAM (PHASEOLUS-MUNGO) SEEDS [J].