CYTOFLUOROMETRIC ANALYSIS OF CHONDROTOXICITY OF FLUOROQUINOLONE ANTIMICROBIAL AGENTS

被引:63
作者
HAYEM, G
PETIT, PX
LEVACHER, M
GAUDIN, C
KAHN, MF
POCIDALO, JJ
机构
[1] HOP CLAUDE BERNARD,INSERM,U13,F-75019 PARIS,FRANCE
[2] CNRS,BIOL FONCTIONNELLE MEMBRANES VEGETALES LAB,F-91190 GIF SUR YVETTE,FRANCE
关键词
D O I
10.1128/AAC.38.2.243
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
To better understand quinolone-related arthropathy, we conceived an experimental ex vivo model using cell cultures of articular chondrocytes issued from pretreated New Zealand White rabbits (NZW). Juvenile (4- to 5-week-old) NZW were orally dosed with ofloxacin or pefloxacin (300 mg/kg of body weight for 1 day) or with pefloxacin (300 mg/kg for 7 days). Adult (5-month-old) NZW were treated with pefloxacin (300 mg/kg for 1 day). Chondrocytes were enzymatically recovered from cartilage and were analyzed by cytofluorometry using 2',7'-dichlorofluorescein diacetate (DCFH-DA) and dihydrorhodamine 123 (DHR), reflecting cellular respiratory-burst activity, and rhodamine 123 (Rh123) and 10-N-nonyl-acridine orange (NAG), specific for the mitochondrial activity and mass, respectively. A significant increase in the respiratory burst was detected by DCFH-DA and DHR in all treated groups of young animals, compared with untreated control groups. No significant increase of respiratory burst was noted in older treated rabbits. The 7-day treatment resulted in a decrease in mitochondrial uptake of Rh123 and an increase in NAO uptake. Fluoroquinolone arthrotoxicity seems to involve in its early phase the respiratory burst of immature articular chondrocytes.
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页码:243 / 247
页数:5
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