ELECTROPHORETIC KARYOTYPE OF MUCOR-CIRCINELLOIDES

被引：19

作者：

NAGY, A

VAGVOLGYI, C

BALLA, E

FERENCZY, L

机构：

[1] ATTILA JOZSEF UNIV,DEPT MICROBIOL,H-6701 SZEGED,HUNGARY

[2] GEDEON RICHTER CHEM WORKS,H-1475 BUDAPEST,HUNGARY

来源：

CURRENT GENETICS | 1994年 / 26卷 / 01期

关键词：

CHEF; ELECTROPHORETIC KARYOTYPE; GENE MAPPING; MUCOR CIRCINELLOIDES;

D O I：

10.1007/BF00326303

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Contour-clamped homogeneous electric field (CHEF) gel electrophoresis was used to separate chromosomal size DNA molecules of two Mucor circinelloides strains. Electrophoretic karyotypes revealed the presence of eight distinct bands for the M. circinelloides f. lusitanicus strain, and four, presumably multiple, bands for the M. circinelloides f. gryseo-cyanus strain. The approximate sizes of the resolved chromosomal DNA bands ranged from 2.3 to 8.1 Mb, giving estimated genome sizes of 38.7 and 32.6 Mb, respectively. Hybridisation techniques were used to assign the leuA gene to a chromosome.

引用

页码：45 / 48

页数：4

共 30 条

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