CHRONIC CANNABINOID ADMINISTRATION ALTERS CANNABINOID RECEPTOR-BINDING IN RAT-BRAIN - A QUANTITATIVE AUTORADIOGRAPHIC STUDY

The active ingredient of marijuana is (-)-DELTA9-tetrahydrocannabinol (DELTA9-THC). DELTA9-THC and other natural and synthetic cannabinoids such as CP-55,940 inhibit spontaneous activity and produce catalepsy in animals in a receptor-mediated fashion. Tolerance develops to the motor effects of DELTA9-THC after repeated administration. To test the hypothesis that tolerance is mediated by changes in cannabinoid receptor binding characteristics, we used quantitative in vitro autoradiography of [H-3]CP-55,940 binding to striatal brain sections from rats treated either chronically or acutely with DELTA9-THC, CP-55,940, or the inactive natural cannabinoid cannabidiol. In the chronic condition, rats were given daily i.p. injections of DELTA9-THC (10 mg/kg), cannabidiol (10 mg/kg), or CP-55,940 (1, 3, or 10 mg/kg) for 2 weeks and sacrificed 30 min after the last injection. In the acute condition, animals received a single dose (10 mg/kg) prior to sacrifice. Rats developed tolerance to the inhibitory effects of DELTA9-THC and CP-55,940, assayed in an open field on days 1, 7, and 14. Cannabidiol had no effect on behavior. Densitometry of [H-3]CP-55,940 binding to brain sections showed that DELTA9-THC- and CP-55,940-treated animals had homogeneous decreases in binding in all structures measured at the selected striatal level. Cannabidiol had no effect on binding. Analysis of binding parameters showed that alterations in the acute condition were attributed to changes in affinity (K(D)), whereas the major changes in the chronic condition were attributed to a lowering of capacity (B(max)). The effects in the 1, 3, and 10 mg/kg CP-55,940 conditions were dose-dependent and paralleled the behavioral data showing that the animals given the highest dose developed the greatest degree of tolerance. The data suggest that tolerance to cannabinoids results at least in part from agonist-induced receptor down-regulation.