RIBONUCLEOTIDYL TRANSFERASE IN PREPARATIONS OF PARTIALLY PURIFIED DNA-POLYMERASE ALPHA OF SEA-URCHIN

被引：4

作者：

MORRIS, PW

RACINE, FM

机构：

[1] Department of Biological Chemistry, University of Illinois Medical Center, Chicago

来源：

NUCLEIC ACIDS RESEARCH | 1978年 / 5卷 / 10期

关键词：

D O I：

10.1093/nar/5.10.3959

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three ribonucleotidyl transferase types have been described in the sea urchin: riboadenylate transferase, the DNA dependent RNA polymerases, and a DNA polymerase associated ribonucleotidyl transferase (Biochemistry 15:3106-3113, 1976). In the present work this latter ribonucleotidyl transferase was found to purify with DNA polymerase α through phosphocellulose, DEAE Sephadex and DNA cellulose and to cosediment t 6.5 S. This ribonucleotidyl transferase was active with Mn+2 , but not Mg+2 on calf thymus DNA and poly(dC). Other synthetic templates elicited DNA polymerase α but no ribonucleotidyl transferase activity. From alkaline hydrolysates of the poly(dC) directed GTP polymerization, we found Goh and Gp in a ratio of 1:16 indicating an average chain length of 17 residues after a 20 min reaction. Co-polymerization of GTP (5 μM) and dGTP (10 μM) yielded a non-random distribution of the ribonucleotide in the deoxyribonucleotide. The properties of this urchin ribonucleotidyl transferase are unlike any previously described eukaryotic transferase and the data is discussed with reference to the known properties of E. coli DNA polymerase I and the primase. © 1978 Information Retrieval Limited.

引用

页码：3959 / 3973

页数：15

共 34 条

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