THE ESCHERICHIA-COLI MU/D108 PHAGE NER HOMOLOG GENE (NLP) IS TRANSCRIBED AND EVOLUTIONARILY CONSERVED AMONG THE ENTEROBACTERIACEAE

被引:10
作者
AUTEXIER, C [1 ]
DUBOW, MS [1 ]
机构
[1] MCGILL UNIV,DEPT MICROBIOL & IMMUNOL,3775 UNIV ST,MONTREAL H3A 2B4,QUEBEC,CANADA
关键词
NER-LIKE PROTEIN; GENE DISRUPTION; SUGAR FERMENTATION; TRANSCRIPTIONAL FUSION; TRANSCRIPTION; MAL GENE EXPRESSION;
D O I
10.1016/0378-1119(92)90701-P
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The Escherichia coli nlp gene is highly homologous to the regulatory ner genes of transposable coliphages, Mu and D108. It was discovered, via its action when overexpressed, as a positive activator of mal gene expression in a cya- crp*1 strain. Chromosomal disruption of the nlp gene by insertion of a promoterless luxAB reporter gene revealed that nlp is nonessential for E. coli viability. Light measurements from the resulting nlp = luxAB transcriptional fusion, plus RNA dot blot analysis, suggest that nlp is transcribed. Southern-blot analyses of DNAs from several bacterial species were performed and indicate that nlp is evolutionarily conserved, but only among closely related Enterobacteriaceae.
引用
收藏
页码:13 / 18
页数:6
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