DETECTION OF MINIMAL RESIDUAL DISEASE STATE IN CHRONIC MYELOGENOUS LEUKEMIA PATIENTS USING FLUORESCENCE IN-SITU HYBRIDIZATION

被引:34
作者
AMIEL, A
YARKONI, S
SLAVIN, S
OR, R
LORBERBOUMGALSKI, H
FEJGIN, M
NAGLER, A
机构
[1] MEIR HOSP,CYTOGENET UNIT,KEFAR SAVA,ISRAEL
[2] HADASSAH UNIV HOSP,DEPT BONE MARROW TRANSPLANTAT,IL-91120 JERUSALEM,ISRAEL
[3] HADASSAH UNIV HOSP,DEPT CELLULAR BIOCHEM,IL-91120 JERUSALEM,ISRAEL
关键词
D O I
10.1016/0165-4608(94)90073-6
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Detection of minimal residual disease and relapse remain major problems in chronic myelogenous leukemia (CML) patients following bone marrow transplantation (BMT). In order to disclose the 9;22 Philadelphia translocation, we used a fluorescence in situ hybridization (FISH) technique. BCR and ABL gene fragments were used as probes for the detection of the BCR/ABL fusion product in peripheral blood and bone marrow cells from 11 CML patients in which 5 were post-BMT The sensitivity and specificity of this approach were compared to conventional cytogenetic and polymerase chain reaction (PCR) methods. FISH demonstrated a high degree of sensitivity (1%) for the detection of the BCR/ABL translocation in these patients. A linear correlation was found between FISH detection of the BCR/ABL fusion product and routine chromosomal analysis (r = 0.995; p < 0.001). Detection of the BCR/ABL signal by FISH was observed in all patients showing a positive PCR signal. A significant reduction in BCR/ABL signal was observed post-transplant (p < 0.001). However, the BCR/ABL translocation was detected in four of five transplanted patients immediately (0.75-2.5 months) following transplant and was found in patients with a low expression of the translocation.
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页码:59 / 64
页数:6
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