ENHANCED EXPRESSION OF P53 MESSENGER-RNA AND PROTEIN IN THE REGRESSING RAT VENTRAL PROSTATE-GLAND

被引：26

作者：

ZHANG, XJ ^{[1
]}

COLOMBEL, M ^{[1
]}

RAFFO, A ^{[1
]}

BUTTYAN, R ^{[1
]}

机构：

[1] HOP HENRI MONDOR,UROL SERV,F-9400 CRETEIL,FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 198卷 / 03期

关键词：

D O I：

10.1006/bbrc.1994.1168

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previously, increased expression of mRNA encoding the p53 tumor suppresor protein was described during castration-induced regression of the rat ventral prostate gland with Northern blot techniques. This activity was confirmed with a ribonuclease protection assay that demonstrated a 16-fold induction of p53 transcripts in ventral prostate RNA within 72 hrs after castration. The induced expression of p53 mRNA correlated with increased detection of p53 protein in nuclei of regressing prostate epithelial cells. Immunohistochemical staining with anti-p53 antibody was strongly reactive for epithelial nuclei in castrated glands but unreactive for nuclei of control adult glands. In contrast to the upregulation of p53 in regressing prostate glands with a large proportion of apoptotic cells, expression of p53 mRNA was decreased in rat prostate glands that were stimulated to regrow by testosterone replacement. © 1994 Academic Press, Inc.

引用

页码：1189 / 1194

页数：6

共 21 条

[1] CELL-PROLIFERATION, DNA-REPAIR, AND P53 FUNCTION ARE NOT REQUIRED FOR PROGRAMMED DEATH OF PROSTATIC GLANDULAR CELLS INDUCED BY ANDROGEN ABLATION [J].