NUCLEASES OF YEAST .I. PROPERTIES AND VARIABILITY OF RIBONUCLEASES

被引:25
作者
NAKAO, Y
LEE, SY
HALVORSON, HO
BOCK, RM
机构
[1] Departments of Bacteriology and Biochemistry, University of Wisconsin, Madison, WI
基金
美国国家科学基金会;
关键词
D O I
10.1016/0005-2744(68)90166-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
1. 1.Nucleases are located in both ribosomes and supernatant of the yeast, Saccharomyces fragilis X Saccharomyces dodzhanskii. Ribosomes prepared from either exponentially growing cells or stationary cells exhibit nuclease activity with a pH optimum of around 7 which is not inhibited by 0.02 M EDTA, Polyvinylsulfate inhibits only at high concentrations. Nuclease activity in the 105 000 × g supernatant from exponentially growing cells has a pH optimum of around 8 and is severely inhibited by EDTA and polyvinylsulfate. In contrast, supernant nuclease from stationary cells has a pH optimum of around 6 and is only partially inhibited by EDTA. 2. 2.Supernant nuclear I and II from logarithmic cells have been partially purified. Their pH optimums are 7.4 and 7.6, respectively. Both nucleases are inhibited by EDTA, Zn2+, polyvinylsulfate and phosphate. The inhibition of EDTA is reversed by the addition of Mg2+. Polyribonucleotides are hydrolysed to di- and trioligonucleotides with 5′-phosphomonoester end groups by both nucleases. © 1968.
引用
收藏
页码:114 / +
页数:1
相关论文
共 16 条
[1]  
ANFINSEN CB, 1954, J BIOL CHEM, V207, P201
[2]   AMINO ACID IMCORPORATION INTO PROTEIN BY CELL-FREE EXTRACTS OF YEAST [J].
BRETTHAUER, RK ;
HALVORSON, HO ;
CHALOUPKA, J ;
BOCK, RM ;
MARCUS, L .
BIOCHEMISTRY, 1963, 2 (05) :1079-&
[3]  
CURRY JB, 1962, BIOCHEM BIOPH RES CO, V6, P415
[4]  
DANNER J, 1963, BIOCHIM BIOPHYS ACTA, V76, P652
[5]  
DAVIDSON JN, 1963, PROGRESS NUCLEIC ED, V2, P83
[6]  
FARKAS WR, 1965, J CELL BIOL, V27, pA27
[7]  
GARVER JC, 1959, J APPL MICROBIOL, V7, P318
[8]  
Gomori G, 1942, J LAB CLIN MED, V27, P955
[9]  
KOERNER JF, 1957, J BIOL CHEM, V228, P1039
[10]   THE NUCLEASES OF ESCHERICHIA-COLI [J].
LEHMAN, IR .
PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY, 1963, 2 :83-123