DERIVATIZATION OF RIBOSOMES AND OF TRANSFER-RNA WITH AN UNDECAGOLD CLUSTER - CRYSTALLOGRAPHIC AND FUNCTIONAL-STUDIES

被引：12

作者：

WEINSTEIN, S

JAHN, W

LASCHEVER, M

ARAD, T

TICHELAAR, W

HAIDER, M

GLOTZ, C

BOECKH, T

BERKOVITCHYELLIN, Z

FRANCESCHI, F

YONATH, A

机构：

[1] MAX PLANCK INST MOLEC GENET,W-1000 BERLIN 33,GERMANY

[2] MAX PLANCK INST MED RES,W-6900 HEIDELBERG 1,GERMANY

[3] EUROPEAN MOLEC BIOL LAB,W-6900 HEIDELBERG,GERMANY

[4] MAX PLANCK RES UNIT RIBOSOMAL STRUCT,HAMBURG,GERMANY

来源：

JOURNAL OF CRYSTAL GROWTH | 1992年 / 122卷 / 1-4期

关键词：

D O I：

10.1016/0022-0248(92)90257-J

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

An undecagold cluster was covalently attached to whole ribosomes and to their small and large subunits prior to their crystallization. X-ray crystallographic data were collected from crystals of the first two. The same cluster was bound to tRNA(phe) from E. coli at base 47. It was found that the modified tRNA molecule binds to the ribosome and can be aminoacylated by its cognate synthetase. The gold cluster modified tRNA(phe) may be used for phasing diffraction data of crystals of complexes containing it, mimicking defined states in the process of protein biosynthesis.

引用

页码：286 / 292

页数：7