7,8-DIHYDRO-8-OXO-2'-DEOXYGUANOSINE PRESENT IN DNA IS NOT SIMPLY AN ARTIFACT OF ISOLATION

被引：36

作者：

HARRIS, G ^{[1
]}

BASHIR, S ^{[1
]}

WINYARD, PG ^{[1
]}

机构：

[1] LONDON HOSP,COLL MED,INFLAMMAT RES GRP,BONE & JOINT RES UNIT,LONDON E1 2AD,ENGLAND

来源：

CARCINOGENESIS | 1994年 / 15卷 / 02期

关键词：

D O I：

10.1093/carcin/15.2.411

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

7,8-Dihydro-8-oxo-2'-deoxyguanosine (8-oxodG) is present in DNA isolated from human and murine cells. Other studies have reported artificially elevated levels in DNA extracted using phenol-based methods. This report shows that DNA, isolated by different methods, with or without the use of a phenol reagent, contains similar levels of 8-oxodG. This, taken with other studies of human tissues which show varying levels of 8-oxodG, indicates that, with due care in the extraction procedure, biologically significant amounts of this altered base are present in mammalian DNA.

引用

页码：411 / 413

页数：3

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