HIGHLY SENSITIVE AND RAPID-DETERMINATION OF THEOPHYLLINE, THEOBROMINE AND CAFFEINE IN HUMAN PLASMA AND URINE BY GRADIENT CAPILLARY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FRIT-FAST ATOM BOMBARDMENT MASS-SPECTROMETRY

被引：27

作者：

HIEDA, Y

KASHIMURA, S

HARA, K

KAGEURA, M

机构：

[1] Department of Forensic Medicine, Fukuoka University School of Medicine, Jonan-ku, Fukuoka, 814-80

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 667卷 / 02期

关键词：

D O I：

10.1016/0378-4347(95)00028-H

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive and reliable analytical procedure has been established for the detection of theophylline (TH), theobromine (TB) and caffeine (CA) in human plasma and urine by gradient capillary high-performance liquid chromatography (HPLC)-frit-fast atom bombardment mass spectrometry (FAB-MS) (LC-frit-FAB-MS), Two capillary columns and a column-switching valve were used in this LC system to allow all of the sample injected to be introduced into the MS system. 7-Ethyltheophylline was used as the internal standard (I.S.). The xanthines in the specimen were extracted with an Extrelut column. The lowest detected amount was ca. 5 ng/ml using this method.

引用

页码：241 / 246

页数：6

共 8 条

[1]

ARAMAKI S, 1991, CHEM PHARM BULL, V39, P2999, DOI 10.1248/cpb.39.2999

[2] THEOPHYLLINE ANALYSIS AT THE CHEST CLINIC - COMPARISON OF A PORTABLE VERSUS CONVENTIONAL SYSTEM [J].