SIMULTANEOUS DETERMINATION OF CAFFEINE AND ITS PRIMARY DEMETHYLATED METABOLITES IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：37

作者：

TANAKA, E

机构：

[1] Institute of Community Medicine, University of Tsukuba, Tsukuba-shi, Ibaraki-ken

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 575卷 / 02期

关键词：

D O I：

10.1016/0378-4347(92)80163-K

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An improved high-performance liquid chromatographic method for the simultaneous determination of caffeine and its three primary metabolites (theophylline, theobromine and paraxanthine) in human plasma is described. The four substances were separated on a reversed-phase column (5-mu-m TSK gel ODS-80TM, 150 mm x 4.6 mm I.D.) by use of the mobile phase methanol-0.1 M NaH2PO4 (30:70, v/v) with a flow-rate of 0.8 ml/min. Absorbance was monitored at 274 nm. The detection limit was 5 ng/ml for theobromine and caffeine and 10 ng/ml for paraxanthine and theophylline. The linearity and reproducibility were sufficient for drug monitoring of caffeine and its primary methylxanthines.

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页码：311 / 314

页数：4

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