DEVELOPMENT AND CHARACTERIZATION OF A RAINBOW-TROUT LIVER-CELL LINE EXPRESSING CYTOCHROME P450-DEPENDENT MONOOXYGENASE ACTIVITY

被引:234
作者
LEE, LEJ [1 ]
CLEMONS, JH [1 ]
BECHTEL, DG [1 ]
CALDWELL, SJ [1 ]
HAN, KB [1 ]
PASITSCHNIAKARTS, M [1 ]
MOSSER, DD [1 ]
BOLS, NC [1 ]
机构
[1] UNIV WATERLOO,DEPT BIOL,WATERLOO N2L 3G1,ON,CANADA
关键词
CYTOCHROME P450; 7-ETHOXYRESORUFIN O-DEETHYLASE; TROUT LIVER CELL LINE;
D O I
10.1007/BF00755606
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
A cell line, RTL-W1, has been developed from the normal liver of an adult rainbow trout by proteolytic dissociation of liver fragments. RTL-W1 can be grown routinely in the basal medium, L-15, supplemented with 5% fetal bovine serum. In this medium, the cells have been passaged approximately 100 times over an 8-year period. The cells do not form colonies ar grow in soft agar. The cultures are heteroploid. The cell shape was predominantly polygonal or epithelial-like, but as cultures became confluent, bipolar or fibroblast-like cells appeared. Among the prominent ultrastructural features of RTL-W1 were distended endoplasmic reticulum and desmosomes. Benzo[a]pyrene was cytotoxic to RTL-W1. Activity for the enzyme, 7-ethoxyresorufin 0-deethylase (EROD), which is a measure of the cytochrome P4501A1 protein, increased dramatically in RTL-W1 upon their exposure to increasing concentrations of either beta-naphthoflavone (BNF) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). With these properties, RTL-W1 should be useful for studying the expression of the cytochrome P450 enzymes and as a tool for assessing the toxic potency of environmental contaminants.
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页码:279 / 294
页数:16
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