NUCLEOTIDE-SEQUENCE AND ANALYSIS OF THE CENTROMERIC REGION OF YEAST CHROMOSOME-IX

被引：22

作者：

VOSS, H ^{[1
]}

TAMAMES, J ^{[1
]}

TEODORU, C ^{[1
]}

VALENCIA, A ^{[1
]}

SENSEN, C ^{[1
]}

WIEMANN, S ^{[1
]}

SCHWAGER, C ^{[1
]}

ZIMMERMANN, J ^{[1
]}

SANDER, C ^{[1
]}

ANSORGE, W ^{[1
]}

机构：

[1] EUROPEAN MOLEC BIOL LAB, BIOL STRUCT & BIOCOMP PROGRAMMES, D-69012 HEIDELBERG, GERMANY

来源：

YEAST | 1995年 / 11卷 / 01期

关键词：

SACCHAROMYCES CEREVISIAE; CHROMOSOME IX; CENTROMERE; NUCLEIC ACID BINDING PROTEINS;

D O I：

10.1002/yea.320110109

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have determined the nucleotide sequence of a cosmid (pIX338) containing the centromere region of yeast (Saccharomyces cerevisiae) chromosome IX. The complete nucleotide sequence of 33.8 kb was obtained by using an efficient directed sequencing strategy in combination with automated DNA sequencing on the A.L.F. DNA sequencer. Sequence analysis revealed the presence of 17 open reading frames (ORFs), four of them previously known yeast genes (sly12, pan1, sts1 andprl1), a tRNA gene and the centromere motif. Exhaustive database searches detected sequence homologues of known function for as many as 14 of the 17 ORFs. These include a mammalian tyrosine kinase substrate; the Escllerichia coli cell cycle protein MinD; the human inositol polyphosphate-5-phosphatase (gene OCRL) involved in Lowe's syndrome, a developmental disorder; and helicases, for which the new yeast member defines a distinct DEAD/H-box subfamily. A surprisingly large fraction of the ORFs (at least six out of 17) in the centromeric region are apparently involved in RNA or DNA binding. The nucleotide sequence reported here has been submitted to the EMBL data library under the accession number X79743.

引用

页码：61 / 78

页数：18

共 82 条

[1]

Abola E. E., 1987, CRYSTALLOGRAPHIC DAT

[2] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].

ALTSCHUL, SF ;

GISH, W ;

MILLER, W ;

MYERS, EW ;

LIPMAN, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410

[3] HIGH-THROUGHPUT AUTOMATED DNA SEQUENCING FACILITY WITH FLUORESCENT LABELS AT THE EUROPEAN MOLECULAR-BIOLOGY LABORATORY [J].

ANSORGE, W ;

VOSS, H ;

WIEMANN, S ;

SCHWAGER, C ;

SPROAT, B ;

ZIMMERMANN, J ;

STEGEMANN, J ;

ERFLE, H ;

HEWITT, N ;

RUPP, T .

ELECTROPHORESIS, 1992, 13 (9-10) :616-619

[4] MAS5, A YEAST HOMOLOG OF DNAJ INVOLVED IN MITOCHONDRIAL PROTEIN IMPORT [J].

ATENCIO, DP ;

YAFFE, MP .

MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (01) :283-291

[5] THE LOWE OCULOCEREBRORENAL SYNDROME GENE ENCODES A PROTEIN HIGHLY HOMOLOGOUS TO INOSITOL POLYPHOSPHATE-5-PHOSPHATASE [J].

ATTREE, O ;

OLIVOS, IM ;

OKABE, I ;

BAILEY, LC ;

NELSON, DL ;

LEWIS, RA ;

MCINNES, RR ;

NUSSBAUM, RL .

NATURE, 1992, 358 (6383) :239-242

[6] THE SWISS-PROT PROTEIN-SEQUENCE DATA-BANK, RECENT DEVELOPMENTS [J].

BAIROCH, A ;

BOECKMANN, B .

NUCLEIC ACIDS RESEARCH, 1993, 21 (13) :3093-3096

[7] RNA-BINDING PROTEINS AS DEVELOPMENTAL REGULATORS [J].

BANDZIULIS, RJ ;

SWANSON, MS ;

DREYFUSS, G .

GENES & DEVELOPMENT, 1989, 3 (04) :431-437

[8] THE PIR-INTERNATIONAL DATABASES [J].

BARKER, W ;

GEORGE, DG ;

MEWES, HW ;

PFEIFFER, F ;

TSUGITA, A .

NUCLEIC ACIDS RESEARCH, 1993, 21 (13) :3089-3092

[9] GENBANK [J].

BENSON, D ;

LIPMAN, DJ ;

OSTELL, J .

NUCLEIC ACIDS RESEARCH, 1993, 21 (13) :2963-2965

[10]

BISSINGER PH, 1994, J BIOL CHEM, V269, P4180

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