NUCLEOTIDE-SEQUENCE AND ANALYSIS OF THE CENTROMERIC REGION OF YEAST CHROMOSOME-IX

被引：22

作者：

VOSS, H ^{[1
]}

TAMAMES, J ^{[1
]}

TEODORU, C ^{[1
]}

VALENCIA, A ^{[1
]}

SENSEN, C ^{[1
]}

WIEMANN, S ^{[1
]}

SCHWAGER, C ^{[1
]}

ZIMMERMANN, J ^{[1
]}

SANDER, C ^{[1
]}

ANSORGE, W ^{[1
]}

机构：

[1] EUROPEAN MOLEC BIOL LAB, BIOL STRUCT & BIOCOMP PROGRAMMES, D-69012 HEIDELBERG, GERMANY

来源：

YEAST | 1995年 / 11卷 / 01期

关键词：

SACCHAROMYCES CEREVISIAE; CHROMOSOME IX; CENTROMERE; NUCLEIC ACID BINDING PROTEINS;

D O I：

10.1002/yea.320110109

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have determined the nucleotide sequence of a cosmid (pIX338) containing the centromere region of yeast (Saccharomyces cerevisiae) chromosome IX. The complete nucleotide sequence of 33.8 kb was obtained by using an efficient directed sequencing strategy in combination with automated DNA sequencing on the A.L.F. DNA sequencer. Sequence analysis revealed the presence of 17 open reading frames (ORFs), four of them previously known yeast genes (sly12, pan1, sts1 andprl1), a tRNA gene and the centromere motif. Exhaustive database searches detected sequence homologues of known function for as many as 14 of the 17 ORFs. These include a mammalian tyrosine kinase substrate; the Escllerichia coli cell cycle protein MinD; the human inositol polyphosphate-5-phosphatase (gene OCRL) involved in Lowe's syndrome, a developmental disorder; and helicases, for which the new yeast member defines a distinct DEAD/H-box subfamily. A surprisingly large fraction of the ORFs (at least six out of 17) in the centromeric region are apparently involved in RNA or DNA binding. The nucleotide sequence reported here has been submitted to the EMBL data library under the accession number X79743.

引用

页码：61 / 78

页数：18

共 82 条

[11] A HOMOLOG OF THE BACTERIAL HEAT-SHOCK GENE DNAJ THAT ALTERS PROTEIN SORTING IN YEAST [J].

BLUMBERG, H ;

SILVER, PA .

NATURE, 1991, 349 (6310) :627-630

[12] DBEST - DATABASE FOR EXPRESSED SEQUENCE TAGS [J].

BOGUSKI, MS ;

LOWE, TMJ ;

TOLSTOSHEV, CM .

NATURE GENETICS, 1993, 4 (04) :332-333

[13] COMPREHENSIVE SEQUENCE-ANALYSIS OF THE 182 PREDICTED OPEN READING FRAMES OF YEAST CHROMOSOME-III [J].

BORK, P ;

OUZOUNIS, C ;

SANDER, C ;

SCHARF, M ;

SCHNEIDER, R ;

SONNHAMMER, E .

PROTEIN SCIENCE, 1992, 1 (12) :1677-1690

[14] AN EXPANDING FAMILY OF HELICASES WITHIN THE DEAD/H SUPERFAMILY [J].

BORK, P ;

KOONIN, EV .

NUCLEIC ACIDS RESEARCH, 1993, 21 (03) :751-752

[15]

BRUNEL C, 1985, PROGR MOL SUBCELLULA

[16] CHARACTERIZATION OF YDJ1 - A YEAST HOMOLOG OF THE BACTERIAL DNAJ PROTEIN [J].

CAPLAN, AJ ;

DOUGLAS, MG .

JOURNAL OF CELL BIOLOGY, 1991, 114 (04) :609-621

[17] DNAJ-LIKE PROTEINS - MOLECULAR CHAPERONES AND SPECIFIC REGULATORS OF HSP70 [J].

CYR, DM ;

LANGER, T ;

DOUGLAS, MG .

TRENDS IN BIOCHEMICAL SCIENCES, 1994, 19 (04) :176-181

[18] TRANSCRIPTION AND REGULATION OF EXPRESSION OF THE ESCHERICHIA-COLI METHIONYL-TRANSFER RNA-SYNTHETASE GENE [J].

DARDEL, F ;

PANVERT, M ;

FAYAT, G .

MOLECULAR & GENERAL GENETICS, 1990, 223 (01) :121-133

[19] THE MIND PROTEIN IS A MEMBRANE ATPASE REQUIRED FOR THE CORRECT PLACEMENT OF THE ESCHERICHIA-COLI DIVISION SITE [J].

DEBOER, PAJ ;

CROSSLEY, RE ;

HAND, AR ;

ROTHFIELD, LI .

EMBO JOURNAL, 1991, 10 (13) :4371-4380

[20] CENTRAL ROLE FOR THE ESCHERICHIA-COLI MINC GENE-PRODUCT IN 2 DIFFERENT CELL DIVISION-INHIBITION SYSTEMS [J].

DEBOER, PAJ ;

CROSSLEY, RE ;

ROTHFIELD, LI .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (03) :1129-1133

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