HETEROGENEITY AMONG PHOTOSYSTEM-I

被引：47

作者：

SVENSSON, P ^{[1
]}

ANDREASSON, E ^{[1
]}

ALBERTSSON, PA ^{[1
]}

机构：

[1] UNIV LUND,CTR CHEM,DEPT BIOCHEM,POB 124,S-22100 LUND,SWEDEN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1060卷 / 01期

关键词：

THYLAKOID MEMBRANE; PHOTOSYSTEM-I; ANTENNA SIZE; PHASE PARTITION; (SONICATION); (SPINACH CHLOROPLAST);

D O I：

10.1016/S0005-2728(05)80117-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thylakoids from spinach were sonicated and separated by aqueous two-phase partitioning into two vesicle populations originating from grana (alpha-vesicles) and stroma lamellae (beta-vesicles), respectively, according to a procedure described earlier (Andreasson et al. (1988) Biochim. Biophys. Acta 936, 339-350). The functional antenna sizes of Photosystem I for these two vesicle populations were determined by measuring the kinetics of the photooxidation of P700. Under green light illumination, the antenna size of Photosystem I of the alpha-vesicles (PSI-alpha) was 40% larger (40% larger rate constant of photooxidation) than that of Photosystem I of the beta-vesicles (PSI-beta). By using actinic light, which is preferentially absorbed by chlorophyll b, this difference was accentuated (60%) indicating that the antenna of PSI-alpha contains relatively more chlorophyll b. The experiments did not support the possibility that this difference in functional antenna size was due to 'spill over' from Photosystem II. A model is presented which shows that PSI-alpha is located in the periphery of the grana and PSI-beta in the stroma lamellae.

引用

页码：45 / 50

页数：6

共 24 条

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