HPLC ANALYSIS OF HEXOSAMINE PHOSPHATES IN BIOLOGICAL SAMPLES

被引：2

作者：

AMBROSIO, S

DEOLIVEIRA, JR

BARTRONS, R

机构：

[1] Unitat de Bioquímica, Facultat Odontologia, Universitat de Barcelona, Barcelona, Campus Universitari de Bellvitge

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1992年 / 25卷 / 04期

关键词：

HPLC; HEXOSAMINE PHOSPHATE; GALACTOSAMINE;

D O I：

10.1016/0165-022X(92)90018-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Galactosamine is quickly metabolized to galactosamine 1-phosphate in rats treated with this compound. An HPLC method to quantify hexosamine phosphates in biological samples is described, modified from the o-phthaldialdehyde amino acid analysis procedure. o-Phthaldialdehyde derivatives of hexosamines and hexosamine-phosphates can be eluted from a reverse-phase column at different retention times, with a total analysis time of 30 min and without overlapping with free amino acids at physiological concentrations. The standard curves are linear between 1 and 40 nmol. This simple method is more selective and sensitive than previous enzymatic analyses of hexosamine phosphorylation.

引用

页码：237 / 244

页数：8

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