SUGAR ANALYSIS OF GLYCOPROTEINS AND GLYCOLIPIDS AFTER METHANOLYSIS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH PULSED AMPEROMETRIC DETECTION

被引:15
作者
LAMPIO, A [1 ]
FINNE, J [1 ]
机构
[1] UNIV TURKU, DEPT MED BIOCHEM, SF-20520 TURKU 52, FINLAND
关键词
D O I
10.1016/0003-2697(91)90368-4
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A procedure for the analysis of the monosaccharide composition of glycoproteins and glycolipids by methanolysis and high-performance liquid chromatography with pulsed amperometric detection is described. The advantage over previous methods is the analysis of underivatized methyl glycosides of all glycoconjugate monosaccharides including sialic acid and uronic acid in a single chromatographic step at the subnanomolar level. © 1991.
引用
收藏
页码:132 / 136
页数:5
相关论文
共 23 条
[1]   GAS CHROMATOGRAPHIC PROPERTIES OF BIOLOGICALLY IMPORTANT N-ACETYLGLUCOSAMINE DERIVATIVES, MONOSACCHARIDES, DISACCHARIDES, TRISACCHARIDES, TETRASACCHARIDES AND PENTASACCHARIDES [J].
BHATTI, T ;
CHAMBERS, RE ;
CLAMP, JR .
BIOCHIMICA ET BIOPHYSICA ACTA, 1970, 222 (02) :339-&
[2]  
Chaplin M.F., 1986, CARBOHYDRATE ANAL, P1
[4]   HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF METHYL GLYCOSIDES [J].
CHEETHAM, NWH ;
SIRIMANNE, P .
JOURNAL OF CHROMATOGRAPHY, 1981, 208 (01) :100-103
[5]   METHANOLYSIS STUDIES OF CARBOHYDRATES, USING HPLC [J].
CHEETHAM, NWH ;
SIRIMANNE, P .
CARBOHYDRATE RESEARCH, 1983, 112 (01) :1-10
[6]  
FINNE J, 1982, METHOD ENZYMOL, V83, P269
[7]   DETERMINATION OF CYCLODEXTRINS AND BRANCHED CYCLODEXTRINS BY REVERSED-PHASE CHROMATOGRAPHY WITH PULSED AMPEROMETRIC DETECTION AND A MEMBRANE REACTOR [J].
HAGINAKA, J ;
NISHIMURA, Y ;
WAKAI, J ;
YASUDA, H ;
KOIZUMI, K ;
NOMURA, T .
ANALYTICAL BIOCHEMISTRY, 1989, 179 (02) :336-340
[8]   SOLUBILIZATION AND COMPARATIVE ANALYSIS OF MAMMALIAN ERYTHROCYTE-MEMBRANE GLYCOPROTEINS [J].
HAMAGUCHI, H ;
CLEVE, H .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1972, 47 (02) :459-+
[9]   MONOSACCHARIDE ANALYSIS OF GLYCOCONJUGATES BY ANION-EXCHANGE CHROMATOGRAPHY WITH PULSED AMPEROMETRIC DETECTION [J].
HARDY, MR ;
TOWNSEND, RR ;
LEE, YC .
ANALYTICAL BIOCHEMISTRY, 1988, 170 (01) :54-62
[10]  
HARDY MR, 1989, METHOD ENZYMOL, V179, P76