DEMONSTRATION OF AMYLOID BETA-PROTEIN SECRETION IN A MOUSE NEURONAL CELL-LINE

被引：12

作者：

ARAKI, W

KUNISHITA, T

TAKAHASHI, K

IKEDA, S

TABIRA, T

机构：

[1] Division of Demyelinating Disease and Aging, National Institute of Neuroscience, NCNP, Kodaira, Tokyo, 187, 4-1-1, Ogawa-higashi

来源：

NEUROSCIENCE LETTERS | 1994年 / 167卷 / 1-2期

关键词：

AMYLOID BETA-PROTEIN; BETA-AMYLOID PRECURSOR PROTEIN; TRANSFECTION; SN49; CELL;

D O I：

10.1016/0304-3940(94)91043-X

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

We investigated the secretion of amyloid beta-protein (beta AP) in a mouse neuronal cell line SN49. SN49 cells stably transfected with mouse beta-amyloid precursor protein (APP) 695 cDNA released approximately three times greater amounts of a 4 kDa protein immunoreactive with anti-beta AP antibodies than untransfected and mock-transfected cells. This 4 kDa protein was further identified as mouse PAP by direct amino acid sequence analysis. These results strongly suggest that the beta AP secretion occurs in mouse neuronal cells as in human cells.

引用

页码：125 / 127

页数：3

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