PURIFICATION AND CHARACTERIZATION OF AN ALKALINE LIPASE FROM PSEUDOMONAS-FLUORESCENS AK102

被引:81
作者
KOJIMA, Y
YOKOE, M
MASE, T
机构
[1] Research and Development Division, Amano Pharmaceutical Co.Ltd, Aichi, Nishikasugai-gun
关键词
D O I
10.1271/bbb.58.1564
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An extracellular, novel alkaline lipase produced by Pseudomonas fluorescens AK102 was purified by ultrafiltration, ammonium sulfate precipitation, and DEAE-Toyopearl 650M and Phenyl-Toyopearl 650M column chromatographies. The purified enzyme was homogeneous on SDS-PAGE. The molecular weight was estimated to be about 33,000 by SDS-PAGE. The isoelectric point was pH 4.0 by isoelectric focusing. The pH stability was 4 to 10 and the optimum pH was 8 to 10. The optimum temperature was 55 degrees C and the enzyme was stable below 50 degrees C. The enzyme unspecifically liberated short chain to long chain fatty acids from p-nitrophenyl esters, methyl esters, and triglycerides. In the presence of an anionic surfactant, the enzyme was characteristically stable. These results suggested that the enzyme can be used as a home laundry product ingredient.
引用
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页码:1564 / 1568
页数:5
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