ONLINE DETERMINATION AND RESOLUTION OF VERAPAMIL ENANTIOMERS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH COLUMN SWITCHING

被引:64
作者
ODA, Y
ASAKAWA, N
KAJIMA, T
YOSHIDA, Y
SATO, T
机构
[1] Department of Physical and Analytical Chemistry, Tsukuba Research Laboratories, Eisai Co., Ltd., Tsukuba-shi, Ibaraki, 300-26
来源
JOURNAL OF CHROMATOGRAPHY | 1991年 / 541卷 / 1-2期
关键词
D O I
10.1016/S0021-9673(01)96013-3
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
High-performance liquid chromatography (HPLC) with column switching was applied to the on-line determination and resolution of the enantiomers of verapamil (VA). This system employs an achiral reversed-phase column coupled to a chiral ovomucoid column via a dilution tube and a trapping column. The reversed-phase column was used to separate VA from the plasma components and impurities, and to determine the total VA concentration using an internal standard method. The eluate containing VA was selectively transferred to the trapping column after suitable dilution with a new mobile phase and VA was concentrated on the trapping column, then passed to the ovomucoid column, where the resolution of enantiomers was performed. It was possible with this system to select independently the optimum mobile phases for both HPLC columns owing to the introduction of the dilution tube and trapping column between them. This method has advantages over the usual HPLC methods in that it is rapid, simple and highly sensitive.
引用
收藏
页码:411 / 418
页数:8
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