UNUSUAL PRODUCT RATIOS RESULTING FROM THE GAMMA-IRRADIATION OF CHOLESTEROL IN LIPOSOMES

被引：18

作者：

MAERKER, G

JONES, KC

机构：

[1] Eastern Regional Research Center, Agriculture Research Service, U.S. Department of Agriculture, Philadelphia, 19118, Pennsylvania

来源：

LIPIDS | 1991年 / 26卷 / 02期

关键词：

D O I：

10.1007/BF02544008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cholesterol in aqueous suspensions of multilamellar vesicles (MLV) was exposed to gamma-irradiation (0.5-10 kGy) at 0-4-degrees-C. Cholesterol oxidation products resulting from the irradiation were isolated by dry column extraction followed by preparative thin-layer chromatography (TLC) and were quantitated by on-column gas chromatography (GC). The ratio of 7-ketocholesterol/cholesterol 5,6-epoxides generated by irradiation was less than one, much lower than the ratio of ten commonly produced by autoxidation. Irradiation also produced relatively higher amounts of 7-hydroxycholesterol than did autoxidation. These unique product ratios may be suitable indicators of past exposure to irradiation.

引用

页码：139 / 144

页数：6

共 36 条

[11] EFFECT OF PH ON THE AFFINITY OF PHOSPHOLIPIDS FOR CHOLESTEROL [J].

JACOBSOHN, MK ;

BAZILIAN, LS ;

HARDIMAN, J ;

JACOBSOHN, GM .

LIPIDS, 1989, 24 (05) :375-382

[12] CORRELATION OF SIDE-CHAIN MOBILITY WITH CHOLESTEROL RETENTION BY PHOSPHOLIPID-VESICLES [J].

JACOBSOHN, MK ;

ESFAHANI, M ;

JACOBSOHN, GM .

LIPIDS, 1986, 21 (11) :691-696

[13]

KIMURA M, 1979, CHEM PHARM BULL, V27, P710

[14] RADIATION-INDUCED REACTIONS OF CHOLESTEROL IN AN AQUEOUS-MEDIUM [J].

KUCERA, J ;

SCHWARZ, V ;

SYKORA, M .

JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, 1984, 87 (04) :219-229

[15] EFFECT OF IONIZING-RADIATION ON CHOLESTEROL IN AQUEOUS DISPERSION [J].

LAKRITZ, L ;

MAERKER, G .

JOURNAL OF FOOD SCIENCE, 1989, 54 (06) :1569-1572

[16]

LEE ML, 1984, OPEN TUBULAR COLUMN, P222

[17] THE OXIDATION OF CHOLESTEROL IN VESICLES [J].

LIJANA, RC ;

MCCRACKEN, MS ;

RUDOLPH, CJ .

BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 879 (02) :247-252

[18] VALIDATION OF A QUANTITATIVE PROCEDURE FOR THE EXTRACTION OF STEROLS FROM EDIBLE OILS USING RADIOLABELED COMPOUNDS [J].

LOGNAY, G ;

DREZE, P ;

WAGSTAFFE, PJ ;

MARLIER, M ;

SEVERIN, M .

ANALYST, 1989, 114 (10) :1287-1291

[19] SPONTANEOUS VESICULATION OF LARGE MULTILAMELLAR VESICLES COMPOSED OF SATURATED PHOSPHATIDYLCHOLINE AND PHOSPHATIDYLGLYCEROL MIXTURES [J].

MADDEN, TD ;

TILCOCK, CPS ;

WONG, K ;

CULLIS, PR .

BIOCHEMISTRY, 1988, 27 (24) :8724-8730

[20] CHOLESTEROL OXIDES .1. ISOLATION AND DETERMINATION OF SOME CHOLESTEROL OXIDATION-PRODUCTS [J].

MAERKER, G ;

UNRUH, J .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1986, 63 (06) :767-771

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