DETERMINATION OF OXYTOCIN IN BIOLOGICAL SAMPLES BY ISOCRATIC HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH COULOMETRIC DETECTION USING C-18 SOLID-PHASE EXTRACTION AND POLYCLONAL ANTIBODY-BASED IMMUNOAFFINITY COLUMN PURIFICATION

被引：16

作者：

KUKUCKA, MA ^{[1
]}

MISRA, HP ^{[1
]}

机构：

[1] VIRGINIA POLYTECH INST & STATE UNIV,VIRGINIA MARYLAND REG COLL VET MED,DEPT BIOMED SCI,BLACKSBURG,VA 24061

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 653卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)E0414-L

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A specific high-performance liquid chromatographic (HPLC) method is described for the reliable quantitation of oxytocin using culture media supernatants. The procedure employs solid-phase extraction, antibody-based immunoaffinity purification and isocratic HPLC with dual channel coulometric detection (ED). The lower limit of detection for this cyclic nonapeptide was 40 pg (40 fmol). Due to its relative simplicity, specificity and precision, the HPLC-ED of oxytocin is an accurate and attractive alternative to many existing quantitative methods.

引用

页码：139 / 145

页数：7

共 17 条

[1]

BENNETT GW, 1986, MONITORING NEUROTRAN, P244

[2]

BRIDGES TE, 1987, LIFE SCI, V41, P285

[3] MONOCLONAL-ANTIBODIES TO OXYTOCIN - PRODUCTION AND CHARACTERIZATION [J].