USE OF ATP, DATP AND THEIR ALPHA-THIO DERIVATIVES TO STUDY DNA-LIGASE ADENYLATION

被引：25

作者：

MONTECUCCO, A ^{[1
]}

LESTINGI, M ^{[1
]}

PEDRALINOY, G ^{[1
]}

SPADARI, S ^{[1
]}

CIARROCCHI, G ^{[1
]}

机构：

[1] CNR,IST GENET BIOCHIM & EVOLUZIONIST,PAVIA,ITALY

来源：

BIOCHEMICAL JOURNAL | 1990年 / 271卷 / 01期

关键词：

D O I：

10.1042/bj2710265

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacteriophage-T4 and human type I DNA ligases were found capable of self-adenylating upon exposure to both ribo- and deoxyribo-[α-35S]thio-ATP. However, the joining reaction does not take place in the presence of the deoxyribotriphosphates. Enzyme adenylation is reversed in all cases by an excess of PP(i), but the rate of reversion is lower with thio derivatives. Therefore thio derivatives can be used to study the adenylation of DNA ligases and to search for specific inhibitors of the first step of the ligation reaction. In addition we show that thio derivatives can be used to detect DNA ligase adenylation activity covalently bound to a solid matrix.

引用

页码：265 / 268

页数：4