CULTURING EMBRYOGENIC PROTOPLASTS OF HAMLIN SWEET ORANGE IN CALCIUM ALGINATE BEADS

被引：24

作者：

NIEDZ, RP

机构：

[1] Agricultural Research Service, U.S. Horticultural Research Laboratory, United States Department of Agriculture, Orlando, 32803, Florida

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1993年 / 34卷 / 01期

关键词：

CA-ALGINATE; CITRUS; PLATING EFFICIENCY; PROTOPLASTS; SOMATIC EMBRYOGENESIS;

D O I：

10.1007/BF00048459

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Protoplasts were enzymatically isolated from log phase embryogenic sweet orange [Citrus sinensis (L.) Osbeck cv. Hamlin] suspension cultures, embedded in 3-mm diameter Ca-alginate beads, and cultured in a growth cabinet (15-20 mu mol m-2 s-1, 4-h photoperiod, 27-degrees-C). Plating efficiency exceeded 90% for Ca-alginate embedded protoplasts vs. 30% for protoplasts cultured in a liquid medium. Embryoids formed from protoplasts were recovered after 20 days by dissolving the Ca-alginate matrix with a calcium sequestrant. Embryoids readily formed shoots that were rooted on MS + 0.01 muM NAA + 5% sucrose. Potential applications are discussed.

引用

页码：19 / 25

页数：7

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