DEGRADATION OF PESTICIDES IN A CONTINUOUS-FLOW 2-PHASE MICROEMULSION REACTOR

被引：10

作者：

KOMIVES, C

OSBORNE, D

RUSSELL, AJ

机构：

[1] UNIV PITTSBURGH,DEPT CHEM & PETR ENGN,PITTSBURGH,PA 15261

[2] UNIV PITTSBURGH,CTR BIOTECHNOL,PITTSBURGH,PA 15261

来源：

BIOTECHNOLOGY PROGRESS | 1994年 / 10卷 / 03期

关键词：

D O I：

10.1021/bp00027a016

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Biocatalytic processes in reversed micelles are limited by the need for continuous reactors in which reaction and separation are feasible. In this paper, we report the design and testing of a continuous-flow centrifugal bioreactor for the enzyme-catalyzed hydrolysis of the common pesticide paraoxon in reverse micelles of Tween 85. In this system, organophosphorus hydrolase catalyzes hydrolysis of the pesticide in an organic-organic two-phase system. The enzyme is retained in one of the phases by encapsulation in reversed micelles, while the other phase is under a continuous flow. This liquid-liquid plug-flow reactor does not leach enzyme because the enzyme is not soluble in the flowing organic phase that contains the paraoxon.

引用

页码：340 / 343

页数：4

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