The Isolation of Eleutherococcus senticosus Constituents by Centrifugal Partition Chromatography and their Quantitative Determination by High Performance Liquid Chromatography

被引:26
作者
Slacanin, Ivan [1 ]
Marston, Andrew [1 ]
Hostettmann, Kurt [1 ]
Guedon, Didier [2 ]
Abbe, Philippe [2 ]
机构
[1] Univ Lausanne, Inst Pharmacognosie & Phytochim, CH-1005 Lausanne, Switzerland
[2] Arkopharma, F-06511 Carros, France
基金
瑞士国家科学基金会;
关键词
Eleutherococcus senticosus; Araliaceae; centrifugal partition chromatography; high performance liquid chromatography; phenolics;
D O I
10.1002/pca.2800020310
中图分类号
Q5 [生物化学];
学科分类号
071010 [生物化学与分子生物学]; 081704 [应用化学];
摘要
An investigation of Eleutherococcus senticosus roots, using centrifugal partition chromatography as a key intermediate separation step, has led to the straightforward isolation of eight constituents. Four of these, sinapaldehyde glucoside, coniferaldehyde glucoside, coniferin and 1,5-di-O-caffeoylquinic acid, have been described for the first time in this plant. The quantitative analysis of different E. senticosus samples has been performed by high performance liquid chromatography using eleutherosides B and E as external standards.
引用
收藏
页码:137 / 142
页数:6
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