IS THERMOTOLERANCE OF YEAST DEPENDENT ON TREHALOSE ACCUMULATION

被引：49

作者：

NWAKA, S ^{[1
]}

KOPP, M ^{[1
]}

BURGERT, M ^{[1
]}

DEUCHLER, I ^{[1
]}

KIENLE, I ^{[1
]}

HOLZER, H ^{[1
]}

机构：

[1] UNIV FREIBURG, INST BIOCHEM, D-79104 FREIBURG, GERMANY

来源：

FEBS LETTERS | 1994年 / 344卷 / 2-3期

关键词：

THERMOTOLERANCE; TREHALOSE; HEAT-SHOCK PROTEIN; HEAT STRESS; NEUTRAL TREHALOSE DELETION MUTANT;

D O I：

10.1016/0014-5793(94)00385-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

During heat stress, trehalose concentration increases in yeast cells in parallel to thermotolerance. This parallelism suggested that trehalose mediated thermotolerance. We show in this work that, under certain conditions, trehalose accumulation and increase in thermotolerance do not go in parallel. A mutant deficient in the trehalose-degrading neutral trehalase shows, after shift from 40 degrees C to 30 degrees C, low thermotolerance in spite of a high trehalose concentration. When glucose is added to stationary yeast cells with high trehalose concentration and high thermotolerance, trehalose concentration decreases while thermotolerance remains high. A mutant deficient in ubiquitin-conjugating genes, ubc4ubc5, shows during exponential growth a low trehalose concentration, but a high thermotolerance, in contrast to wild-type cells. Because the ubc4ubc5 mutant synthesizes heat-shock proteins constitutively, it is proposed that, under these conditions, accumulation of heat-shock proteins, and not trehalase, mediates thermotolerance.

引用

页码：225 / 228

页数：4

共 14 条

[1] TREHALOSE ACCUMULATES IN SACCHAROMYCES-CEREVISIAE DURING EXPOSURE TO AGENTS THAT INDUCE HEAT-SHOCK RESPONSE [J].